硝酸镧浸种对盐胁迫下棉花种子萌发及幼苗生理特性的影响  

Effects of Soaking Seeds with Lanthanum Nitrate on Seed Germination and Seedling Physiological Characteristics of Cotton under Salt Stress

作　　者：蒋建勋 徐婧 刘晓燕 张承礼 曹平平 王安录 郭维新[1] 张忠波[1] 冯国艺[4] JIANG Jian-xun;XU Jing;LIU Xiao-yan;ZHANG Cheng-li;CAO Ping-ping;WANG An-lu;GUO Wei-xin;ZHANG Zhong-bo;FENG Guo-yi(Cangzhou Academy of Agriculture and Forestry Sciences,Cangzhou 061001,China;Hebei Seed Station,Shijiazhuang 050031,China;Cangzhou Modern Agricultural and Rural Development Promotion Center,Cangzhou 061001,China;Institute of Cotton,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China)

机构地区：[1]沧州市农林科学院,河北沧州061001 [2]河北省种子总站,河北石家庄050031 [3]沧州市现代农业农村发展促进中心,河北沧州061001 [4]河北省农林科学院棉花研究所,河北石家庄050051

出　　处：《江西农业学报》2024年第6期15-20,共6页Acta Agriculturae Jiangxi

基　　金：国家现代农业产业技术体系(CARS-15-18);河北省现代农业产业技术体系建设专项资金(HBCT2024100404);河北省棉花现代种业科技创新团队(21326314D)。

摘　　要：以棉花种子为试材,采用种子发芽箱培养法,探究了不同浓度(0、25、50、75、100 mg/L)硝酸镧溶液浸种对150 mmol/L氯化钠胁迫下棉花种子萌发的性状和幼苗抗氧化酶活性、渗透调节物质含量的影响。结果表明:(1)150 mmol/L氯化钠抑制棉花种子萌发,幼苗的抗氧化酶活性和可溶性蛋白含量降低,可溶性糖含量、丙二醛含量升高;(2)50 mg/L硝酸镧浸种对盐胁迫下棉花种子萌发的促进效果最显著,其发芽势、发芽率、发芽指数、胚根和胚芽长度以及生物量均显著增加;(3)硝酸镧浸种可增加盐胁迫下棉花幼苗抗氧化酶活性,随着硝酸镧浸种浓度的升高,POD、SOD和CAT含量呈先升高后降低的变化趋势,MDA含量先降低后升高且在硝酸镧浓度为50 mg/L时抗氧化酶活性最强;(4)盐胁迫下棉花幼苗的可溶性糖、可溶性蛋白质和脯氨酸含量随着硝酸镧浓度的增加呈先升高后降低的变化趋势,硝酸镧浓度为50 mg/L时渗透调节物质含量最高。综上,硝酸镧浸种可提高棉花种子萌发及幼苗生长的耐盐性,浓度为50 mg/L的促进效果最显著。Cotton seeds were taken as research materials in this experiment,and seed germination box culture method was used to study the effects of lanthanum nitrate solution with different concentrations(0,25,50,75 and 100 mg/L)on seed germination characteristics,antioxidant enzyme activity and osmoregulatory substance content under 150 mmol/L NaCl stress.The results showed that:(1)When NaCl was 150 mmol/L,cotton seed germination was inhibited,antioxidant enzyme activity and soluble protein content of cotton seeding decreased,and soluble sugar content and malondialdehyde content increased.(2)Soaking with 50 mg/L lanthanum nitrate had the most significant effect on the germination of cotton seeds under salt stress,with a significant increase in the germination potential,germination rate,germination index,embryonic root length,germination length and biomass.(3)Soaking with lanthanum nitrate could increase the activities of antioxidant enzymes during seeding growth under salt stress.With the increase of lanthanum nitrate concentration,the contents of POD,SOD and CAT first increased and then decreased,and the content of MDA first decreased and then increased.The antioxidant enzyme activities were strongest at a concentration of 50 mg/L.(4)The contents of soluble sugar,soluble protein and proline first increased and then decreased during germination of cotton seeding under salt stress when the concentration of lanthanum nitrate increased.The content of osmoregulatory substance was the highest when the concentration of lanthanum nitrate was 50 mg/L.In conclusion,soaking with lanthanum nitrate can improve the tolerance to salt stress during the germination period of cotton seeds and seeding growth,and the effect was the most significant when the concentration was 50 mg/L.

关 键 词：棉花 硝酸镧 盐胁迫 种子萌发 幼苗 生理特性 

分 类 号：S562[农业科学—作物学]