28种中药及其复方对奶牛乳房炎病原菌金黄色葡萄球菌的体外抑菌试验  

In vitro Antibacterial Test of 28 Traditional Chinese Medicines and Their Compounds against the Pathogenic Dacteria Staphylococcus aureus of Dairy Cow Mastitis

作　　者：鲁俊廷 LU Jun-ting(Qixia Animal Disease Prevention and Control Center,Qixia 265300)

机构地区：[1]山东省栖霞市动物疫病预防控制中心,栖霞265300

出　　处：《中国奶牛》2024年第7期46-50,共5页China Dairy Cattle

摘　　要：为筛选对奶牛乳房炎病原菌金黄色葡萄球菌具有抑菌作用的中药,并分析部分中药联合的抑菌效果,试验采用水提法提取28种中药有效成分,使其药物浓度为1g/mL;在采用试管二倍稀释法和平板打孔法分别测定28种中药抑菌效果的基础上,选取对金黄色葡萄球菌具有高度敏感和极度敏感的中药进行两两组合后,采用微量肉汤稀释棋盘法测定双联中药复方的MIC值,并计算双联中药复方FIC指数。结果显示,28种中药中17种中药对金黄色葡萄球菌表现出不同程度的抑菌效果,其中金银花、诃子、蒲公英和甘草4种中药对金黄色葡萄球菌的抑菌活性较为突出,抑菌圈≥15mm,MIC7.81~15.63mg/mL,MBC15.63~31.25mg/mL;诃子+蒲公英、金银花+蒲公英和金银花+诃子3种双联中药复方对金黄色葡萄球菌的FIC指数分别为0.75、0.75和1.00,主要表现为相加作用;金银花+甘草、诃子+甘草和蒲公英+甘草3种双联中药复方对金黄色葡萄球菌的FIC指数分别为1.50、1.50和2.00,主要表现为无关作用。以上结果表明,用对金黄色葡萄球菌抑菌效果较好的中药组成双联中药复方后,其抑菌效果会增强。To screen traditional Chinese medicine that has antibacterial effects on the pathogenic bacteria Staphylococcus aureus of dairy cow mastitis,and analyze the antibacterial effects of some combinations of traditional Chinese medicine.The experiment used water extraction method to extract the effective ingredients of 28 traditional Chinese medicines,with a drug concentration of 1 g/mL;On the basis of measuring the antibacterial effects of 28 traditional Chinese medicines using the test tube double dilution method and the plate drilling method,a pair of highly sensitive and extremely sensitive traditional Chinese medicines to Staphylococcus aureus were selected for combination.The MIC value of the double traditional Chinese medicine compound was measured using the micro broth dilution chessboard method,and the FIC index of the double traditional Chinese medicine compound was calculated.The results showed that 17 out of 28 traditional Chinese medicines exhibited varying degrees of antibacterial effects on Staphylococcus aureus.Among them,Honeysuckle,Terminalia chebula,Dandelion,and Glycyrrhiza uralensis had more prominent antibacterial activity against Staphylococcus aureus,with a inhibition zone of 15 mm,MIC7.81-15.63mg/mL,and MBC15.63-31.25mg/mL;The FIC indices of the three double combination traditional Chinese medicine formulas of Chezi+Dandelion,Honeysuckle+Dandelion,and Honeysuckle+Chezi against Staphylococcus aureus were 0.75,0.75,and 1.00,respectively,mainly manifested as additive effects;The FIC indices of three dual combination Chinese herbal formulas,namely honeysuckle+licorice,chebula+licorice,and dandelion+licorice,on Staphylococcus aureus were 1.50,1.50,and 2.00,respectively,indicating an unrelated effect.The above results indicate that the antibacterial effect of a dual combination Chinese medicine compound composed of traditional Chinese medicine with good antibacterial effect on Staphylococcus aureus will be enhanced.

关 键 词：奶牛乳房炎 金黄色葡萄球菌 中药 体外抑菌 分级抑菌浓度(FIC) 

分 类 号：S858.23[农业科学—临床兽医学]