植物药组方MYF-20的抗马拉色菌活性及初步作用机制探究  

Inhibitory effects and preliminary mechanism of the medicinal botanical formula MYF-20 against Malassezia

作　　者：杨帆 周代红 茆灿泉[1] YANG Fan;ZHOU Daihong;MAO Canquan(School of Life Science and Engineering,Southwest Jiaotong University,Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs,Chengdu 610031,China)

机构地区：[1]西南交通大学生命科学与工程学院,四川省天然药物仿生合成工程研究中心,成都610031

出　　处：《中国真菌学杂志》2024年第3期229-234,共6页Chinese Journal of Mycology

基　　金：四川省科技厅科技计划重点项目(2022YFS0437)。

摘　　要：目的以糠秕马拉色菌(Malassezia furfur)为目标菌株,对本实验室研发的植物药组方MYF-20的乙醇提取物进行抗菌活性及初步作用机制研究。方法采用改良CLSI-M27A法确定组方对马拉色菌的最小抑菌浓度,电导率法以及扫描电镜形态学观察研究MYF-20对马拉色菌细胞完整性的影响,铜皂法检测组方对马拉色菌脂肪酶活性影响,构建大蜡螟感染模型评价MYF-20的体内药效。结果MYF-20对马拉色菌的最小抑菌浓度为7.83 mg/mL(生药干重),MYF-20处理后,菌液相对电导率显著上升(P<0.01);扫描电镜证实了菌体芽孢断裂、细胞破裂内陷的发生;脂肪酶活性被显著抑制(P<0.01)。大蜡螟体内实验证明,MYF-20可以显著提高大蜡螟马拉色菌感染模型的存活率与黑化健康评分,降低体内真菌负荷(P<0.01)。结论MYF-20对马拉色菌在体内外均具有强抗菌活性,可通过多途径发挥其抑菌功能,具有成为抗马拉色菌植物类新药的一定潜力。Objective Inhibitory effects and preliminary mechanism of the ethanol extracts of the medicinal botanical formula MYF-20 innovatively developed in biological laboratory against Malassezia furfur were conducted in this research.Methods The modified CLSI-M27A method was applied to determine the minimum inhibitory concentration of the formula against Malassezia furfur,and scanning electron microscopy morphology was used to learn the effect of MYF-20 on the cell integrity of Malassezia furfur,detecting the effect of the MYF-20 on the lipase activity of Malassezia furfur by method of copper soap.Moreover,infection model of Galleria mellonella was constructed to evaluate the antifungal activity of MYF-20 in vivo.Results The minimum inhibitory concentration of MYF-20 for Malassezia furfur was 7.83 mg/mL(crude dry weight of the botanical medicine drug).Furthermore,MYF-20 could significantly increase the relative conductivity of the Malassezia solution(P<0.01),scanning electron microscopy further confirmed the occurrence of spore fracture and cell rupture concavity.Meanwhile,it also significantly inhibited the activity of lipase(P<0.01).Insect experiments demonstrated that MYF-20 could significantly enhance the survival rate and melanization score of Galleria mellonella after infected with Malassezia and reduce the fungal burden in vivo(P<0.01).Conclusions MYF-20 has strong anti-Malassezia activity both in vitro and in vivo,and could exert its anti-Malassezia function through multiple ways,which had the potential to be developed as a new anti-Malassezia botanical drug.

关 键 词：植物药 MYF-20 马拉色菌 大蜡螟 抗菌活性 

分 类 号：R379.9[医药卫生—病原生物学]