Large‑scale transcriptomic and genomic analyses reveal a novel functional gene SERPINB6 for chicken carcass traits  

作　　者：Di Zhao Ranran Liu Xiaodong Tan Huimin Kang Jie Wang Zheng Ma Haiquan Zhao Hai Xiang Zhengfen Zhang Hua Li Guiping Zhao 

机构地区：[1]Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,School of Life Science and Engineering,Foshan University,Foshan,China [2]State Key Laboratory of Animal Biotech Breeding,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing,China [3]Guangdong Tinoo’s Foods Group Co.,Ltd.,Qingyuan,China

出　　处：《Journal of Animal Science and Biotechnology》2024年第4期1466-1481,共16页畜牧与生物技术杂志（英文版）

基　　金：supported by grants from the Key Projects of National Natural Science Foundation of China (No. 32230101);the Project of Qingyuan Science and Technology (2020A01, 2021SJXM011);the Agriculture Research System (CARS-41);the Core Breed Source Research Project JBGS (2021) 107

摘　　要：Background Carcass traits are crucial indicators of meat production efficiency.However,the molecular regulatory mechanisms associated with these traits remain unclear.Results In this study,we conducted comprehensive transcriptomic and genomic analyses on 399 Tiannong partridge chickens to identify key genes and variants associated with carcass traits and to elucidate the underlying regulatory mechanisms.Based on association analyses with the elastic net(EN)model,we identified 12 candidate genes(AMY1A,AP3B2,CEBPG,EEF2,EIF4EBP1,FGFR1,FOXD3,GOLM1,LOC107052698,PABPC1,SERPINB6 and TBC1D16)for 4 carcass-related traits,namely live weight,dressed weight,eviscerated weight,and breast muscle weight.SERPINB6 was identified as the only overlapping gene by 3 analyses,EN model analysis,weighted gene co-expression network analysis and differential expression analysis.Cell-level experiments confirmed that SERPINB6 promotes the proliferation of chicken DF1 cells and primary myoblasts.Further expression genome-wide association study and association analysis indicated that rs317934171 is the critical site that enhances SERPINB6 expression.Furthermore,a dual-luciferase reporter assay proved that gga-miR-1615 targets the 3′UTR of SERPINB6.Conclusions Collectively,our findings reveal that SERPINB6 serves as a novel gene for chicken carcass traits by promoting fibroblast and myoblast proliferation.Additionally,the downstream variant rs317934171 regulates SERPINB6 expression.These results identify a new target gene and molecular marker for the molecular mechanisms of chicken carcass traits.

关 键 词：Carcass traits CHICKEN Genome SERPINB6 TRANSCRIPTOME 

分 类 号：S831.2[农业科学—畜牧学]