tRNA^(Glu)-derived fragments from embryonic extracellular vesicles modulate bovine embryo hatching  

作　　者：Yuan Fan Krishna Chaitanya Pavani Katrien Smits Ann Van Soom Luc Peelman 

机构地区：[1]Department of Veterinary and Biosciences,Faculty of Veterinary Medicine,Ghent University,Heidestraat 19,9820 Merelbeke,Belgium [2]Department of Internal Medicine,Reproduction and Population Medicine,Faculty of Veterinary Medicine,Ghent University,Salisburylaan 133,9820 Merelbeke,Belgium [3]Department for Reproductive Medicine,Ghent University Hospital,Corneel Heymanslaan 10,9000 Ghent,Belgium

出　　处：《Journal of Animal Science and Biotechnology》2024年第4期1559-1566,共8页畜牧与生物技术杂志（英文版）

基　　金：supported by Ghent University(Grant:Bijzonder Onderzoeksfonds Geconcerteerde Onderzoeksactie 2018000504[GOA030-18 BOF]);supported by Ghent University:BOF.STG.2022.02.0034.01;supported by China Scholarship Council:Grant 202006910034;supported by Fonds Wetenschappelijk Onderzoek:Grant 1228821N and 12A2H24N。

摘　　要：Transfer RNA-derived small RNAs(tsRNAs)have been shown to be involved in early embryo development and repression of endogenous retroelements in embryos and stem cells.However,it is unknown whether tsRNAs also regulate embryo hatching.In this study,we mined the sequencing data of a previous experiment in which we demonstrated that the microRNA(miRNA)cargo of preimplantation embryonic extracellular vesicles(EVs)influences embryo development.We thus profiled the tsRNA cargo of EVs secreted by blastocysts and non-blastocysts.The majority of tsRNAs was identified as tRNA halves originating from the 5'ends of tRNAs.Among the 148 differentially expressed tsRNAs,the 19 nt tRNA fragment(tRF)tDR-14:32-Glu-CTC-1 was found to be significantly up-regulated in EVs derived from non-blastocysts.RT-qPCR assays confirmed its significant up-regulation in non-blastocyst embryos and their conditioned medium compared to the blastocyst group(P<0.05).Inhibition of tDR-14:32-Glu-CTC-1 by supplementing antagomirs to the conditioned medium improved embryo hatching(P<0.05).Transcriptomic analysis of embryos treated with tDR-14:32-Glu-CTC-1 antagomirs further showed differential expression of genes that are associated with embryo hatching and implantation.In summary,tDR-14:32-Glu-CTC-1 is up-regulated in non-blastocyst embryos and their secretions,and inhibition of tDR-14:32-Glu-CTC-1 promotes embryo hatching,while influencing embryo implantation-related genes and pathways.These results indicate that embryonic EVs containing specific tRFs may regulate preimplantation embryo development.

关 键 词：EMBRYO Extracellular vesicles HATCHING tRNA fragments ts RNAs 

分 类 号：S823.3[农业科学—畜牧学]