浙江8份特异刺葡萄种质资源植物学特征及扦插特点分析  

Botanical and Cutting Characteristics Analysis of Eight Specific Vitis Davidii Germplasm Resources in Zhejiang region

作　　者：郑婷[1] 魏灵珠[1] 罗飞雄 郑伟尉[3] 向江 梅建平 吴江[1] 程建徽[1] ZHENG Ting;WEI Lingzhu;LUO Feixiong;ZHENG Weiwei;XIANG Jiang;MEI Jianping;WU Jiang;CHENG Jianhui(Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Horticulture,Hunan Agricultural University,Changsha 410128,China;College of Agriculture and Food Science,Zhejiang A&F,Hangzhou 311300,China;Agricultural and Rural Bureau of Jinyun,Jinyun 321499,China)

机构地区：[1]浙江省农业科学院园艺研究所,浙江杭州310021 [2]湖南农业大学园艺学院,湖南长沙410128 [3]浙江农林大学园艺科学学院,浙江杭州311300 [4]浙江省缙云县农业农村局,浙江缙云321499

出　　处：《中外葡萄与葡萄酒》2024年第4期53-58,共6页Sino-Overseas Grapevine & Wine

基　　金：浙江省“三农九方”科技协作计划(2024SNJF026);浙江省果品新品种选育重大专项(2021C02066-6);财政部和农业农村部:国家现代农业产业技术体系(CARS-29-13)。

摘　　要：刺葡萄原产中国,是东亚种群的特色野生资源之一,通过SSR分子标记分析刺葡萄的遗传多样性,对其物候期、植物学特性和生根特性等进行评价。本研究共筛选出8份浙江特异种质资源标记为VD1、VD3、VD5、VD6、VD9、VD11、VD12和VD15,聚类分析显示刺葡萄有着很强的地域性;刺葡萄生根困难,在无任何加温等措施下,VD15生根率最高,为13.33%,愈伤组织诱导率为86.67%;切片结果表明,皮刺的位置表皮细胞延伸增多,葡萄愈伤组织形成与生根是两个独立的过程。以上结果为刺葡萄资源的筛选和利用奠定了基础。Vitisdavidii is one of the characteristic wild grape resources of East Asian populations,originating from China.The genetic diversity was analyzed,using SSR molecular markers,and their phenological,botanical,and rooting characteristics were evaluated.This study screened 8 specific germplasm resources in Zhejiang region,including VD1,VD3,VD5,VD6,VD9,VD11,VD12,and VD15.Cluster analysis showed that Vitisdavidii have strong regional characteristics.The rooting of Vitisdavidii was difficult,without any heating or other measures,VD15 had the best cutting rooting,which had 13.33%of rooting rate,and 86.67%of callus induction rate.The slicing results showed that the epidermal cells at the location of the prickles extended more.The formation of grape callus tissue and rooting were two independent processes.The above results have laid the foundation for the screening and utilization ofVitisdavidii resources.

关 键 词：刺葡萄 SSR 扦插 生根 

分 类 号：S663.1[农业科学—果树学]