亚致死剂量氯虫苯甲酰胺对稻水象甲生物酶活性及田间发育动态的影响  

Effects of sublethal doses of chlorantraniliprole on the enzyme activity and field development dynamics of Lissorhoptrus oryzophilus

作　　者：杨春林 胡强 李洪浩[3] 席亚东[4] YANG Chunlin;HU Qiang;LI Honghao;XI Yadong(Leshan Plant Protection and Quarantine Station,Leshan,Sichuan 614000,China;Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province,Leshan Normal University,Leshan,Sichuan 614000,China;Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610066,China;The Institute of Plant Protection,Sichuan Academy of Agricultural Sciences/Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province,Chengdu,Sichuan 610066,China)

机构地区：[1]乐山市植保植检站,四川乐山614000 [2]乐山师范学院竹类病虫防控与资源开发四川省重点实验室,四川乐山614000 [3]四川省农业科学院,四川成都610066 [4]四川省农业科学院植物保护研究所/蔬菜品种改良与种质创新四川省重点实验室,四川成都610066

出　　处：《生物安全学报（中英文）》2024年第3期274-284,共11页Journal of biosafety

基　　金：四川省科技计划项目(2021YFYZ0021);四川省农业科学院科技成果中试熟化与示范转化项目(2023ZSSFXD02);国家现代农业产业体系四川创新团队项目(川农函[2019]472号);乐山市重点科技计划项目(14NZD141)。

摘　　要：【目的】了解氯虫苯甲酰胺对稻水象甲的亚致死效应,为水稻生产中使用氯虫苯甲酰胺科学高效防治稻水象甲提供参考。【方法】以越冬代稻水象甲成虫为试虫,通过测定不同种群(RW-Js、RW-Gy、RW-Mz、RW-Zy、RW-Lc)稻水象甲对氯虫苯甲酰胺的敏感性,确定氯虫苯甲酰胺对稻水象甲的致死中浓度(LC_(50))和亚致死剂量(LC_(25));分别采用室内测定和田间调查的方法,研究亚致死剂量氯虫苯甲酰胺对稻水象甲体内多种生物酶活性及田间发育动态的影响。【结果】不同来源稻水象甲种群对氯虫苯甲酰胺的敏感性差异较大,RW-Js种群的LC_(50)值为RW-Gy种群的13.45倍,达到中等抗性水平;解毒酶抑制剂胡椒基丁醚(piperomyl butoxide,PBO)对氯虫苯甲酰胺的协同增效作用明显高于磷酸三苯酯(triphenyl phosphate,TPP)和顺丁烯二酸二乙酯(diethyl maleate,DEM);抗性种群RW-Js体内3种解毒酶活性较敏感种群RW-Gy显著增高(P<0.05),其中多功能氧化酶(mixed function oxidase,MFO)变化最为明显。稻水象甲成虫经氯虫苯甲酰胺LC_(25)剂量处理后,虫体内几种生物酶活性总体呈先升高后降低的趋势,具明显的时间效应,其中MFO活性在整个测定周期内均显著高于对照处理(P<0.05);氯虫苯甲酰胺LC_(25)剂量对稻水象甲各虫态的田间始见期、发生高峰期和发生末期均产生不同程度的影响。【结论】解毒酶活性可能与稻水象甲对氯虫苯甲酰胺的敏感性有关,MFO是稻水象甲对氯虫苯甲酰胺解毒代谢过程中最重要的酶,在抗性形成中起主要作用;亚致死剂量氯虫苯甲酰胺可延缓稻水象甲幼虫、蛹和一代成虫的生长发育,缩短成虫寿命、加快田间成虫消亡。【Aim】This study aimed to understand the sublethal effect of chlorantraniliprole on rice water weevil(Lissorhoptrus oryzophilus Kusehel)and to provide reference for the scientific and efficient control of L.oryzophilus Kusehel using chlorantraniliprole in rice production.【Method】Using the overwintering L.oryzophilus adults as test insects,the median lethal concentration(LC_(50))and sublethal dose(LC_(25))of chlorantraniliprole to populations RW-Js,RW-Gy,RW-Mz,RW-Zy,and RW-Lc were determined by measuring the sensitivity of L.oryzophilus to chlorantraniliprole.The effects of sublethal doses of chlorantraniliprole on the activities of various biological enzymes and field development dynamics of Lissorhoptrus oryzophilus were studied using indoor measurements and field investigations,respectively.【Result】Significant differences were noted in the sensitivity of different L.oryzophilus populations to chlorantraniliprole,with the LC 50 value of RW-Js population being 13.45 times that of RW-Gy population,reaching a moderate resistance level.The synergistic effect of the detoxification enzyme inhibitor PBO on chlorantraniliprole was significantly greater than that of TPP and DEM.The activities of the three detoxifying enzymes in the resistant population,RW-Js,were significantly higher than those in the sensitive population,RW-Gy(P<0.05),with the most significant change observed in multifunctional oxidase(MFO).The activities of several biological enzymes in the L.oryzophilus treated with LC_(25) dose of chlorantraniliprole increased first and then decreased,and the activity of MFO was significantly higher than that of the control treatment throughout the entire measurement period(P<0.05).The LC 25 dose of chlorantraniliprole had a certain degree of impact on the initial,peak,and final stages of L.oryzophilus occurrence in the field.【Conclusion】The activity of detoxification enzyme is possibly related to the sensitivity of L.oryzophilus to chlorantraniliprole.MFO is the most important detoxification enzyme in

关 键 词：稻水象甲 氯虫苯甲酰胺 亚致死剂量 生物酶活性 解毒酶抑制剂 发育动态 

分 类 号：S435.112.6[农业科学—农业昆虫与害虫防治]