不同氮磷营养条件对中肋骨条藻和球等鞭金藻生长及元素组成的影响  

Effects of Different Nitrogen and Phosphorus Nutrient Conditions on Growth and Element Composition of Skeletonema costatum and Isochrysis galbana

作　　者：凌晨 付发武 张翠霞 雷灵逸[4] 金书言 刘小芳 张守庆 鲁翠翠 Ling Chen;Fu Fawu;Zhang Cuixia;Lei Lingyi;Jin Shuyan;Liu Xiaofang;Zhang Shouqing;Lu Cuicui(College of Marine and Environmental Science,Tianjin University of Science&Technology,Tianjin 300457,China;Tianjin Marine Environmental Protection and Restoration Technology Engineering Center,Tianjin 300457,China;Tianjin Key Laboratory of Marine Resources and Chemistry,Tianjin 300457,China;College of Environment&Safety Engineering,Fuzhou University,Fuzhou 350108,China;Institute for Advanced Marine Research,China University of Geosciences,Guangzhou 511462,China;Zhongke Blue Ocean(Tianjin)Technology Company Limited,Tianjin 300450,China)

机构地区：[1]天津科技大学海洋与环境学院,天津300457 [2]天津市海洋环境保护与修复技术工程中心,天津300457 [3]天津市海洋资源与化学重点实验室,天津300457 [4]福州大学环境与安全工程学院,福建福州350108 [5]中国地质大学,广州南沙地大滨海研究院,广州南沙511462 [6]中科蓝海(天津)科技有限公司,天津300450

出　　处：《中国海洋大学学报（自然科学版）》2024年第8期63-73,共11页Periodical of Ocean University of China

基　　金：国家自然科学基金青年基金项目(41306119);天津科技大学实验室创新基金项目(1705A303)资助。

摘　　要：为了探究氮磷比(N/P)失衡和不同藻种对海洋微藻的生长和元素组成及营养利用的影响,本研究分别在正常条件(f/2培养基)、低N/P条件(氮限制)和高N/P条件(磷限制)下,连续培养了中肋骨条藻(Skeletonema costatum)和球等鞭金藻(Isochrysis galbana)两种微藻各15 d,培养过程中测定了两种微藻的细胞密度、叶绿素a浓度、藻细胞的元素含量(C、N、P)和化学计量比,以及培养液中无机盐的含量。结果表明:氮限制条件促进两种微藻的增殖,而磷限制条件抑制了两种微藻增殖;营养失衡不利于藻细胞叶绿素a的积累;在不同N/P条件下两种藻的C含量变化较小,而随着时间推移,两种藻细胞的N和P元素含量均有所降低,且在两种藻细胞的高N/P组中N含量均达到最高,不同N/P条件对细胞N元素含量有极显著影响;两种藻细胞在低N/P组和高N/P组中的P含量均同对照组无显著性差异;不同藻种对细胞C元素和P元素的含量有显著性影响;中肋骨条藻在高N/P组和低N/P组的C/N值、在低N/P组的C/P值以及在高N/P组的N/P值均同对照组存在显著性差异;球等鞭金藻在三种条件下的各元素比值均与对照组无明显差异;不同N/P条件只对细胞N/P值有极显著影响;氮限制增加了NO_(3)^(-)-N的利用率,磷限制降低了NO_(3)^(-)-N的利用率,而这两种营养失衡均对藻细胞利用PO _(4)^(3-)-P的影响很小。In this paper,two species of microalgae,Skeletonema costatum and Isochrysis galbana were cultured continuously for 15 days under three different nutritional conditions:Normal condition(f/2 medium),low N/P condition(nitrogen limitation)and high N/P condition(phosphorus limitation).During the culture process,the cell density,chlorophyll a concentration,element content(C,N,P)and stoichiometric ratio of algal cells and the content of inorganic salts in the culture medium were measured.The effects of N/P imbalance and different algae species on the growth,elemental composition and nutrient utilization of marine microalgae were investigated.The results showed that:(1)nitrogen-limiting conditions promoted the proliferation of two microalgae,phosphorus-limiting conditions reduced the proliferation of microalgae;nutrient imbalance was not conducive to the accumulation of Chlorophyll a in algal cells;(2)Under different N/P conditions,the two the C content of the algae changed little,the contents of N and P in the cells of the two algae decreased with time,and the content of N was the highest in the high N/P group.Different N/P conditions had a very significant effect on the content of N in the cells;There was no significant difference in P content between the low N/P group and the high N/P group and the control group;Different algae species had significant effects on the contents of C and P elements in cells;(3)For Skeletonema costatum,the C/N value in high N/P group and low N/P group,the C/P value in low N/P group and the N/P value in high N/P group were all significantly different from the control group;For Isochrysis galbana,there was no significant difference in the ratios of various elements between the three conditions and the control group;Different N/P conditions only had a very significant effect on cell N/P value;(4)Nitrogen limitation increased the utilization of NO_(3)^(-)-N,and phosphorus limitation decreased the utilization of NO_(3)^(-)-N;however,these two nutrient imbalances have little effect on the utiliz

关 键 词：中肋骨条藻 球等鞭金藻 f/2培养基 高氮磷比条件 低氮磷比条件 

分 类 号：P735[天文地球—海洋生物学]