西沙海绵来源共附生真菌Penicillium sclerotiorum次级代谢产物研究  

Studies on the Secondary Metabolites of a Sponge-Derived Fungus Penicillium sclerotiorum from the Xisha Islands

作　　者：李晓蕾 杨亚楠 唐旭利[2] 李国强[1] Li Xiaolei;Yang Yanan;Tang Xuli;Li Guoqiang(Key Laboratory of Marine Medicine,Ministry of Education,College of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]中国海洋大学化学化工学院,山东青岛266100

出　　处：《中国海洋大学学报（自然科学版）》2024年第8期98-102,共5页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(U2006204,41776136,2181101213,81991522)资助。

摘　　要：本文对1株西沙群岛来源土黄孔海绵共附生真菌Penicillium sclerotiorum进行了次级代谢产物研究。利用硅胶柱层析、薄层色谱、Sephadex LH-20凝胶色谱、中压制备液相色谱(MPLC)和高效液相色谱(HPLC)等分离手段对菌株发酵提取物进行分离和纯化。从乙酸乙酯提取物中共分离出1个新的甾醇(1)、1个新的苯衍生物(4)和4个已知化合物。通过核磁共振(NMR)、质谱(MS)、文献数据比较并结合生物合成途径确定了这些化合物的平面结构和绝对构型。The authors investigated the secondary metabolites of a sponge-derived fungi Penicillium sclerotiorum from Xisha Islands.The fermentation extract of the strain was separated and purified by silica gel column chromatography,thin layer chromatography,Sephadex LH-20 chromatography,medium pressure liquid chromatography(MPLC)and high performance liquid chromatography(HPLC).A total of one new sterol(1),one new benzene derivative(4)and four known compounds were isolated from the ethyl acetate extract.The structures and absolute configurations of the isolates were established based on nuclear magnetic resonance(NMR),mass spectrometry(MS),literature comparison and combined with the biosynthetic pathway.

关 键 词：西沙群岛 海绵共附生真菌 次级代谢产物 甾醇类化合物 细胞毒活性 

分 类 号：R931.77[医药卫生—生药学]