膝骨关节炎不同证候患者关节液BMP-2和CTGF浓度特点及其与证候程度间的相关性研究  

Study on the Concentration Characteristics of BMP-2 and CTGF in Synovial Fluid of Patients with Different Syndromes of Knee Osteoarthritis and Its Correlation with the Degree of Syndrome

作　　者：王乾顺 刘向春[1] 刘叶 亓建[1] 吴红飞 刘勤哲 刘宸 刘雨煊 叶超[1] WANG Qianshun;LIU Xiangchun;LIU Ye;QI Jian;WU Hongfei;LIU Qinzhe;LIU Chen;LIU Yuxuan;YE Chao(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Institute of Rehabilitation Information,China Institute of Rehabilitation Sciences,Beijing 100068,China)

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]中国康复科学所康复信息研究所,北京100068

出　　处：《辽宁中医药大学学报》2024年第8期39-43,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82104710)。

摘　　要：目的研究膝骨关节炎肝肾亏虚证和痰瘀互结证患者关节液骨形态发生蛋白-2(BMP-2)和结缔组织生长因子(CTGF)的浓度特点及其与证候程度间的相关性。方法留取并测定89例膝骨关节炎不同证候患者关节液BMP-2和CTGF的浓度,应用中医证候评分量表量化证候程度,分析关节液BMP-2和CTGF的浓度特点及其与证候程度间的相关性。结果膝骨关节炎痰瘀互结证和肝肾亏虚证患者年龄、身高、体质量、生命体征、病程比较上差异未见统计学意义(P>0.05);膝骨关节炎肝肾亏虚证患者关节液BMP-2的浓度水平显著高于痰瘀互结证患者(z=-2.212,P<0.05);膝骨关节炎肝肾亏虚证患者关节液CTGF的浓度水平显著低于痰瘀互结证患者(z=-2.072,P<0.05);膝骨关节炎肝肾亏虚证患者关节液CTGF浓度水平与中医证候评分呈负相关(r^(2)=0.240,P<0.05)。结论膝骨关节炎痰瘀互结证和肝肾亏虚证患者关节液BMP-2和CTGF浓度特点不同,肝肾亏虚证患者关节液CTGF浓度可以在一定程度上反映其证候的严重程度,上述发现与中医病因病机的分析相一致,其作用机制可能与转化生长因子β(TGF-β)/Smad信号通路的激活程度不同有关。Objective To study the concentration characteristics of BMP-2 and CTGF in patients with knee osteoarthritis,liver-kidney deficiency syndrome and stagnation of phlegm and blood stasis syndrome,and their correlation with the degree of syndrome.Methods The concentrations of BMP-2 and CTGF in the joint fluid of 89 patients with different symptoms of knee osteoarthritis were collected and measured,and the concentration characteristics of BMP-2 and CTGF in joint fluid and their correlation with the degree of syndrome were analyzed by applying the medical syndrome score scale.Results There was no significant statistical differences in age,height,weight,vital signs and course of patients between patients with stagnation of liver-kidney deficiency syndrome in knee osteoarthritis and stagnation of phlegm and blood stasis syndrome;(P>0.05);The concentration level of joint fluid BMP-2 in patients with knee osteoarthritis and liver-kidney deficiency syndrome was significantly higher than that in patients with stagnation of phlegm and blood stasis syndrome(z=-2.212,P<0.05).The concentration level of CTGF in joint fluid in patients with knee osteoarthritis liver-kidney deficiency syndrome was significantly lower than that in patients with stagnation of phlegm and blood stasis syndrome(z=-2.072,P<0.05);The CTGF concentration level of joint fluid in patients with knee osteoarthritis liver-kidney deficiency syndrome was negatively correlated with the TCM syndrome score(r^(2)=0.240,P<0.05).Conclusion The concentration of BMP-2 and CTGF in patients with knee osteoarthritis with liver-kidney deficiency syndrome and stagnation of phlegm and blood stasis syndrome is different,and the concentration of CTGF in joint fluid in patients with liver-kidney deficiency syndrome can reflect the severity of the syndrome to a certain extent,which is consistent with the analysis of the etiology and pathogenesis of traditional chinese medicine,and its mechanism of action may be related to the activation degree of TGF-β/Smad signaling pathway.

关 键 词：膝骨关节炎 证候 骨形态发生蛋白-2 结缔组织生长因子 关节液 肝肾亏虚证 痰瘀互结证 

分 类 号：R274[医药卫生—中医骨伤科学]