番茄SlHDT3基因在调控干旱和盐胁迫耐受力中的功能分析  

Functional analysis of tomato SlHDT3 gene in regulating drought and salt stress tolerance

作　　者：郭俊娥 王辉辉 张彬[3] GUO Jun'e;WANG Huihui;ZHANG Bin(Department of Biological and Food Engineering,Lyuliang University,Lüliang,Shanxi 033001,China;Department of of Chemical and Material Engineering,Lyuliang University,Lüliang,Shanxi 033001,China;College of Agriculture,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China)

机构地区：[1]吕梁学院生物与食品工程系,山西吕梁033001 [2]吕梁学院化工与材料工程系,山西吕梁033001 [3]山西农业大学农学院,山西晋中030801

出　　处：《植物生理学报》2024年第6期976-988,共13页Plant Physiology Journal

基　　金：山西省基础研究计划(202103021224319和202103021224322);吕梁市农业领域重点研发项目(2022NYGG30)。

摘　　要：组蛋白去乙酰化酶基因在植物生长发育、果实成熟以及胁迫响应中起着至关重要的作用。为揭示SlHDT3基因在番茄干旱和盐胁迫耐受力中的作用,对野生型番茄幼苗进行非生物胁迫与植物生长调节物质处理,利用qRT-PCR技术分析其对SlHDT3相对表达水平的影响,发现SlHDT3参与多种非生物胁迫及植物生长调节物质处理的响应。为了进一步研究SlHDT3的详细功能,对SlHDT3进行沉默载体构建,并利用农杆菌介导技术成功转入番茄外植体中,对野生型和SlHDT3-RNAi植株进行干旱和盐胁迫处理。结果表明:沉默SlHDT3后进行干旱和盐处理,SlHDT3-RNAi植株复水存活率更低、叶绿素降解更快,脯氨酸含量、过氧化氢酶活性及ABA浓度降低,丙二醛含量和H3水平乙酰化组蛋白量升高。转录组分析数据显示,胁迫相关基因的表达出现了不同程度的上调与下调。结果初步明确了SlHDT3作为正向调控因子在番茄植株的干旱胁迫和盐胁迫耐受力起作用,为番茄分子育种提供了理论基础。Histone deacetylase(HDACs)genes perform as crucial regulators widely participation in the process of plant growth development,ripening and stress response in plants.To revealed the role of tomato SlHDT3 gene under drought and salt stress tolerance,wild-type tomato seedlings were subjected to abiotic stress and plant growth regulators treatment and the relative expression level of SlHDT3 were analyzed by qRT-PCR.It was found that SlHDT3 was involved in the response to various abiotic stresses and plant growth regulators treatments.An RNAi vector was constructed and transferred successfully into wild-type tomato for further research on the detailed functions of the histone deacetylation gene SlHDT3.The result suggested that silencing SlHDT3,there was no significant difference in the growth of WT and Sl-HDT3-RNAi under normal conditions.After drought and salt treatment,the survival rate of SlHDT3-RNAi plants was lower,chlorophyll degradation was faster,proline content,CAT activity and ABA concentration were decreased,while MDA content and total histone H3 acetylation level were increased.The results of RNA-seq analysis data showed that varying degrees of up-regulated or down-regulated of stress-related genes were occur in SlHDT3-RNAi plants compared with the WT.The results suggest that SlHDT3 acts as a stress-responsive gene,plays a role in the positive regulation of abiotic stress tolerance,and may be one of the new target genes in the engineering breeding of salt-and drought-tolerant tomato.

关 键 词：番茄 SlHDT3 脱落酸 干旱胁迫 盐胁迫 

分 类 号：S641.2[农业科学—蔬菜学]