醛固酮诱导的主动脉夹层小鼠血浆和主动脉组织基质金属蛋白酶-2表达及意义  

Expression and significance of matrix metalloproteinase-2 in plasma and aortic tissue of mice with aldosterone induced aortic dissection

作　　者：党毓婕 朱晴[2] 李南方[2] DANG Yujie;ZHU Qing;LI Nanfang(Graduate School,Xinjiang Medical University,Urumqi,Xinjiang Uygur Autonomous Region 830054,China;Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region,Xinjiang Hypertension Institute,NHC Key Laboratory of Hypertension Clinical Research,Key Laboratory of Xinjiang Uygur Autonomous Region"Hypertension Research Laboratory"Xinjiang Clinical Medical Research Center for Hypertension(Cardio-Cerebrovascular)Diseases,Urumqi,Xinjiang Uygur Autonomous Region 830001,China)

机构地区：[1]新疆医科大学研究生院,新疆维吾尔自治区乌鲁木齐830054 [2]新疆维吾尔自治区人民医院高血压病中心、新疆高血压病研究所、国家卫生健康委员会高血压临床研究重点实验室、新疆维吾尔自治区重点实验室"高血压研究实验室"、新疆高血压(心脑血管)病临床医学研究中心,新疆维吾尔自治区乌鲁木齐830001

出　　处：《中华实用诊断与治疗杂志》2024年第7期663-667,共5页Journal of Chinese Practical Diagnosis and Therapy

基　　金：“天山英才”培养计划科技创新团队项目(2023TSYCTD0016)。

摘　　要：目的 观察醛固酮诱导的主动脉夹层小鼠血浆及主动脉组织中基质金属蛋白酶(MMP)-2表达,探讨其与ALD诱导的主动脉夹层的关系。方法 45只C57BL/6J雄性小鼠随机分为对照组、模型组、治疗组各15只。模型组和治疗组每日泵注醛固酮500μg/kg,饮用质量分数0.9%氯化钠+质量分数0.2%氯化钾溶液,连续4周;治疗组自泵注醛固酮第2天每日给予螺内酯20 mg/kg灌胃,连续4周。对照组不做处理,正常进食水。3组分别于造模前、造模第2周、造模结束后测量尾部血压;测量血压后全身麻醉,眼内眦静脉采血,采用ELISA法检测血浆MMP-2水平;采血后处死取腹部主动脉组织,行HE染色及Masson染色观察主动脉组织形态变化和纤维化情况,采用免疫荧光法检测主动脉组织MMP-2蛋白表达量。结果 (1)3组造模前、造模第2周、造模结束后收缩压比较差异均无统计学意义(P>0.05),造模前、造模第2周舒张压比较差异均无统计学意义(P>0.05),造模结束后舒张压比较差异均有统计学意义(P<0.05)。模型组造模结束后舒张压高于对照组、治疗组(P<0.05),对照组与治疗组比较差异无统计学意义(P>0.05)。(2)HE染色结果显示,模型组小鼠主动脉壁内膜和中膜破裂、受损,血液灌注假腔及滋养血管形成;对照组和治疗组小鼠主动脉组织基本正常。Masson染色结果显示,模型组小鼠主动脉全层均有胶原蛋白沉积,弹力纤维排列紊乱;治疗组血管胶原沉积明显减少;对照组小鼠主动脉结构紧凑,胶原纤维连续。(3)模型组造模结束后血浆MMP-2水平[(0.29±0.13)ng/L]及主动脉组织MMP-2蛋白表达量(37.79±2.28)均高于对照组[(0.26±0.14)ng/L、26.90±2.86]、治疗组[(0.26±0.01)ng/L、29.32±2.48](P<0.05),对照组与治疗组比较差异均无统计学意义(P>0.05)。结论 醛固酮诱导的主动脉夹层小鼠血浆和主动脉组织MMP-2表达上调、主动脉组织受损;拮抗醛固酮后MMP-2表达下调、主Objective To observe the expression of matrix metalloproteinase-2(MMP-2) in plasma and aortic tissue of mice with aldosterone induced aortic dissection, and to investigate its relationship with aortic dissection induced by aldosterone.Methods Forty-five male C57BL/6J mice were randomly divided into control group,model group and treatment group,with 15mice in each group.Model group and treatment group received pump of 500μg/kg aldosterone daily,and oral administration of 0.9% NaCl+ 0.2% KCl solution,totally for 4consecutive weeks.Treatment group received spironolactone(20mg/kg)by gavage on the next day after aldosterone pump,totally for 4consecutive weeks.Control group received normal intaking water with no treatment.The tail blood pressure was measured before,at week 2and at the end of modeling in three groups,respectively.ELISA was used to detect the plasma level of MMP-2from the inner canthal vein under general anesthesia in three groups.The abdominal aortic tissues were taken after execution.HE staining and Masson staining were used to observe the morphological changes and fibrillation of the aortic tissues,and immunofluorescence method was used to detect the expression of MMP-2protein.Results(1)There was no significant difference in the systolic blood pressure among three groups before,at week 2and at the end of modeling(P>0.05).The diastolic blood pressure showed no significant difference before and at week 2of modeling(P>0.05).At the end of modeling,the diastolic blood pressure showed significant difference among three groups(P<0.05),was higher in model group than that in control group and treatment group(P<0.05),and showed no significant difference between control group and treatment group(P>0.05).(2)The HE staining results showed that the aortic intima and media in model group were ruptured and damaged,leading to the formation of blood perfusion false lumens and nourishing blood vessels.The aortic tissues in control and treatment groups were basically normal.The Masson staining results showed collagen de

关 键 词：主动脉夹层 醛固酮 螺内酯 基质金属蛋白酶-2 C57BL/6J雄性小鼠 

分 类 号：R543.1[医药卫生—心血管疾病]