沉默Periostin基因调节小鼠成骨细胞代谢对抑制破骨活性的作用  

Silencing of Periostin gene modulates murine osteoblast metabolism to inhibit bone resorption activity

作　　者：蔡军 秦晗 CAI Jun;QIN Han(Department of Anesthesiology,Maternal and Child Health Hospital of Lianyungang Affiliated to Yangzhou University,Lianyungang 222006,Jiangsu,China)

机构地区：[1]扬州大学附属连云港市妇幼保健院麻醉科,江苏连云港222006 [2]徐州医科大学附属连云港医院口腔医院儿童口腔科,江苏连云港222002

出　　处：《广东医学》2024年第7期819-823,共5页Guangdong Medical Journal

基　　金：国家自然科学基金项目(81500893);连云港市卫生健康面上科技项目(202225)。

摘　　要：目的通过观察沉默Periostin后小鼠成骨细胞中特异性转录因子(Runx2)、核因子-κB受体活化因子配体(RANKL)及骨保护素(OPG)表达变化,探讨其影响破骨活性的分子机制。方法将小鼠成骨细胞株(MC3T3-E1)分为实验组和对照组,实验组使用LVpFU-GW-016PSC66473-1病毒转染,对照组用pFU-GW-016PSC53349-1病毒,保持两组细胞状态相当。用MDC法检测基因沉默效果,Western blot法检测两组细胞Runx2、RANKL和OPG蛋白表达情况。结果沉默Periostin后发现:实验组荧光表达较对照组明显增强(P<0.01);实验组Runx2和RANKL蛋白表达较对照组明显下调(P<0.05);OPG蛋白表达较对照组显著升高(P<0.05)。结论沉默Periostin可改变小鼠成骨细胞RANKL和OPG表达,并可能通过核因子-κB(NF-κB)信号通路介导影响Runx2基因,增强成骨细胞功能并抑制骨破坏。Objective To investigate the molecular mechanisms by which silencing of the Periostin gene affects the expression of Runx2,receptor activator of nuclear factor-kappa B ligand(RANKL),and osteoprotegerin(OPG)in murine osteoblasts and its impact on bone resorption activity.Methods Murine osteoblast cell line MC3T3-E1 was divided into an experimental group and a control group.The experimental group was transfected with LVpFU-GW-016PSC66473-1 virus,while the control group received pFU-GW-016PSC53349-1 virus to ensure equivalent cellular conditions.The efficiency of gene silencing was assessed using MDC method,and the protein expression of Runx2,RANKL,and OPG in both groups was evaluated by Western blotting.Results Following Periostin silencing,the experimental group showed significantly enhanced fluorescence expression compared to the control group(P<0.01).Protein expression of Runx2 and RANKL was significantly decreased(P<0.05),whereas OPG expression was markedly increased(P<0.05)in the experimental group compared to the control group.Conclusion Silencing of Periostin alters RANKL and OPG expression in murine osteoblasts,potentially influencing the Runx2 gene via the nuclear factor-kappa B(NF-κB)signaling pathway.This enhancement of osteoblast function may contribute to the inhibition of bone resorption.

关 键 词：PERIOSTIN 特异性转录因子 核因子-kB受体活化因子配体 骨保护素 成骨细胞 骨代谢 

分 类 号：R318.04[医药卫生—生物医学工程] Q254[医药卫生—基础医学]