微量法筛血在Rh血型系统相容性输血中的应用研究  

Application Value of Micro-screening in Compatible Transfusion of Rh Blood Group System

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作  者:陈晓霞[1] 舒群峰 刘冬[1] 周吉[1] 彭冬菊[1] 焦丹梅 Chen Xiaoxia;Shu Qunfeng;Liu Dong;Zhou Ji;Peng Dongju;Jiao Danmei(Shiyan Center Blood Station,Shiyan HuBei 442000,China)

机构地区:[1]十堰市中心血站,湖北十堰442000

出  处:《襄阳职业技术学院学报》2024年第4期116-118,共3页Journal of Xiangyang Polytechnic

摘  要:目的研究快速筛选患者Rh血型系统C、c、E和e意外抗体对应抗原阴性血液的方法以及该方法在相容性输血中的价值。方法文章以产生意外抗-e的O型患者为研究对象,使用微孔板稀释献血者红细胞,制备2%~5%红细胞悬液,用微量法检测标本红细胞Rh血型抗原,用标准法确定目标血液导管Rh血型抗原,比较两种方法的差异性。结果使用微量法检测的献血者标本Rh血型抗原结果与标准法确认结果100%相符,临床无溶血性输血反应发生。结论在不影响细胞凝集和结果观察的前提下,微量法可以节约近70%的试剂、耗材成本,且该方法简便易行,为临床产生Rh血型系统意外抗体患者的精准输血治疗赢取宝贵时间,适合基层血站和输血科开展应用。Objective To study the method of rapid screening of Rh blood group system C,c,E,and e unexpected antibody corresponding antigen negative blood in patients,and the value of this method in compatible blood transfusion.Method This article takes the patient with type O who developed unexpected anti e as an example.The red blood cells of the sample were diluted using a microplate to detect red blood cells,and a 2%to 5%red blood cell suspension was prepared.The Rh blood group antigen of the sample red blood cells was detected using a micro test tube method,and the target blood duct Rh blood group antigen was determined using a standard test tube method.The differences between the two methods were compared.Result The Rh blood group antigen results of donor specimens were 100%consistent with the confirmed results of blood catheter specimens,and there was no hemolytic transfusion reaction in clinical practice.Conclusion The combination of microplate and test tube micro method can save nearly 70%of reagent and consumable costs without affecting the observation of cell agglutination results.Moreover,this method is simple and easy to implement,providing valuable time for precise blood transfusion treatment of patients with Rh blood group system unexpected antibodies in clinical practice.It is suitable for application in grassroots blood stations and transfusion departments.

关 键 词:RH血型 意外抗体 输血 

分 类 号:R457[医药卫生—治疗学]

 

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