毛乌素沙地不同恢复年限柠条固沙林微生物群落特征及其影响因素  

Microbial community characteristics and their influencing factors in Caragana korshinskii sand-fixing forests of different restoration years in the Mu Us Desert,China

作　　者：杨钧 王瑞霞 王俊 于双 杨博[1] 王文强 杨君珑 李小伟[1,3] YANG Jun;WANG Ruixia;WANG Jun;YU Shuang;YANG Bo;WANG Wenqiang;YANG Junlong;LI Xiaowei(College of Forestry and Pratacuture,Ningxia University,Yinchuan 750021,China;Ningxia Lingwu Baijitan National Nature Reserve Administration,Lingwu 750400,Ningxia,China;State Key Laboratory Breeding Base of Land Degradation and Ecological Restoration of Northwest China,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学林业与草业学院,银川750021 [2]宁夏灵武白芨滩国家级自然保护区管理局,宁夏灵武750400 [3]宁夏大学,西北土地退化与生态恢复省部共建国家重点实验室培育基地,银川750021

出　　处：《应用生态学报》2024年第7期1807-1814,共8页Chinese Journal of Applied Ecology

基　　金：宁夏自然科学基金项目(2022AAC03080);宁夏高等学校一流学科建设(草学学科)项目(NXYLXK2017A01);宁夏草原监测项目(2023)资助。

摘　　要：土壤微生物是连接地上植被和土壤养分循环的关键枢纽,然而对于毛乌素沙地生态恢复过程中植被和土壤对微生物群落的影响还不明确。本研究以流动沙丘为对照(0年),以10、30、50和70年的柠条固沙林土壤为研究对象,将磷脂脂肪酸(PLFA)标记法与灌木、草本植物和土壤因子结合,探究不同恢复年限下土壤微生物群落特征的变化规律及其调控因子。结果表明:随着恢复年限的增加,生态恢复效应指数显著增加。0、10、30、50和70年的总PLFA含量分别为47.75、55.89、63.53、67.23和82.29 nmol·g^(-1)。随着生态恢复指数增加,细菌含量和革兰氏阳性菌与革兰氏阴性菌比均显著增加;革兰氏阴性菌群落含量和真菌与细菌比均显著下降。灌木、草本植物和土壤因子能解释土壤微生物群落72.4%的变异,其中土壤因子的贡献高于植被因子。毛乌素沙地土壤微生物群落的总PLFA含量随恢复年限的增加而增加,并且土壤含水量、酸碱度、全氮和土壤有机碳为影响土壤微生物群落特征的主要驱动因子。随着毛乌素沙地柠条固沙林恢复年限的增加,主要由土壤因子驱动的土壤微生物群落结构发生显著变化。Soil microorganisms play a crucial role as a link between vegetation and soil nutrient cycling.However,it is unclear how vegetation and soil influence microbial community during the ecological restoration process of the Mu Us Desert.Using phospholipid fatty acid(PLFA)markers and integrating shrub,herbaceous plants,and soil factors,we explored the characteristics and regulations of soil microbial community changes.In this study,we used and took the soil after 10,30,50,and 70 years of Caragana korshinskii sand-fixing forest restoration,with moving dunes as a control(0 year).The results showed that the ecological restoration effect index increased significantly with the increase of recovery years.The total PLFA contents in 0,10,30,50,and 70 years were 47.75,55.89,63.53,67.23,and 82.29 nmol·g^(-1),respectively.With the increases of ecological restoration index,the biomass of fungi and bacteria,as well as the ratio of Gram-positive to Gram-negative bacteria,all showed significant increase,while the biomass of Gram-positive and Gram-negative bacterial communities,and the ratio of fungi to bacteria,demonstrated significant decrease.Shrub,herbaceous plants,and soil factors could explain 72.4% of the vari-ation of soil microbial community composition,with higher contribution of soil factors than vegetation factors.The total content of phospholipid fatty acids of soil microbial community in Mu Us Desert increased with the increases of restoration years.Soil water content,pH,total nitrogen,and soil organic carbon were the main driving factors affecting the characteristics of soil microbial community.With the increases of restoration years of C.korshinskii sand-fixation forests in the Mu Us Desert,there were significant changes in the structure of soil microbial communities,which were primarily driven by soil factors.

关 键 词：土壤微生物 磷脂脂肪酸 毛乌素沙地 植被恢复 

分 类 号：S714.3[农业科学—林学]