七叶皂苷对结直肠癌细胞增殖、侵袭的作用及机制研究  

Effect and Mechanism of Escin on Proliferation and Invasion of Colorectal Cancer Cells

作　　者：习隽丽 周超 李子银 石磊[2] XI Junli;ZHOU Chao;LI Ziyin;SHI Lei(Department of Gastroenterology,The Third Hospital of Wuhan,Wuhan 430074,China;Department of OncologyⅢ,People′s Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉市第三医院消化内科,武汉430074 [2]武汉大学人民医院肿瘤三科,武汉430060

出　　处：《世界中医药》2024年第13期1954-1958,1965,共6页World Chinese Medicine

基　　金：国家自然科学基金面上项目(81871816);湖北省卫生健康委员会面上项目(WJ2021M157);湖北省自然科学基金项目(2020CFB661);武汉市卫生健康委员会面上项目(WX20B14);武汉大学医学部教学研究项目(2020028)。

摘　　要：目的:探究七叶皂苷对氧化三甲胺(TMAO)诱导的结直肠癌细胞株增殖及迁移侵袭的影响,并探讨其作用机制。方法:取结直肠癌HCT116细胞系,分为对照组、TMAO组、观察组3组,并分别进行处理。对照组不做处理,TMAO组仅使用100μmol/L TMAO诱导,观察组使用同剂量TMAO诱导后再进行10μmol/L七叶皂苷治疗处理。随后使用四甲基偶氮唑盐比色(MTT)法、划痕愈合实验及细胞迁移侵袭试验技术(Transwell)实验分别检测细胞的增殖、迁移及侵袭行为,并使用蛋白质印迹(Western Blotting)实验检测SRC、pBTK、pTRIO蛋白表达情况。结果:TMAO诱导可增强HCT116增殖及迁移侵袭能力,并显著上调SRC-BTK-TRIO通路相关基因的mRNA水平,而七叶皂苷干预能抑制癌细胞增殖(P<0.01)并降低其迁移(P<0.001)、侵袭(P<0.01)能力,同时下调SRC(P<0.05)、BTK(P<0.01)、TRIO(P<0.05)蛋白的表达。结论:七叶皂苷可通过下调SRC-BTK-TRIO通路显著抑制HCT116细胞增殖及迁移侵袭能力。Objective:To investigate the effect of escin on the proliferation,migration,and invasion of colorectal cancer cell line HCT116 induced by trimethylamine N-oxide(TMAO) and to explore its mechanism of action.Methods:HCT116 colorectal cancer cells were divided into a control group,a TMAO group,and an experimental group.The control group received no treatment,the TMAO group was induced with 100 μmol/L TMAO,and the experimental group was treated with 100 μmol/L TMAO followed by 10 μmol/L escin.Cell proliferation,migration,and invasion were assessed using the MTT assay,scratch wound healing assay,and Transwell migration and invasion assays,respectively.The expression levels of SRC,pBTK,and pTRIO proteins were measured by Western blotting.Results:TMAO induction enhanced the proliferation,migration,and invasion of HCT116 cells and significantly upregulated the mRNA levels of genes in the SRC-BTK-TRIO pathway.Escin treatment inhibited cancer cell proliferation(P<0.01),reduced their migration(P<0.001) and invasion(P<0.01) capabilities,and downregulated the expression of SRC(P<0.05),BTK(P<0.01),and TRIO(P<0.05) proteins.Conclusion:Escin can significantly inhibit the proliferation,migration,and invasion of HCT116 cells by downregulating the SRC-BTK-TRIO pathway.

关 键 词：七叶皂苷 结直肠癌 增殖 迁移 侵袭 SRC BTK TRIO 

分 类 号：R284[医药卫生—中药学]