阴虚胃痛胶囊对慢性萎缩性胃炎大鼠胃黏膜超微结构和炎症介质的影响  

Effects of Yinxu Weitong Capsules on the Ultrastructure and Inflammatory Mediators of Gastric Mucosa in Rats with Chronic Atrophic Gastritis

作　　者：张晨晨 李丹艳 朱泠霏 李吉磊 刘念 张声生[2] ZHANG Chenchen;LI Danyan;ZHU Lingfei;LI Jilei;Liu Nian;ZHANG Shengsheng(Beijing University of Chinese Medicine,Beijing 100029,China;Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China)

机构地区：[1]北京中医药大学,北京100029 [2]首都医科大学附属北京中医医院,北京100010

出　　处：《世界中医药》2024年第14期2106-2112,共7页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(82004332);国家中医药管理局中医传承与创新“百千万”人才工程(岐黄学者)—国家中医药领军人才支撑计划项目(国中医药人教函[2021]203号)。

摘　　要：目的:观察阴虚胃痛胶囊对慢性萎缩性胃炎(CAG)大鼠胃黏膜超微结构及炎症介质的影响。方法:将72只无特定病原体(SPF)级斯泼累格·多雷(SD)大鼠分为正常组13只和造模组59只,造模组给予N-甲基-N′-硝基-N-亚硝基胍自由饮+灌胃,同时给予饥饱失常法复合因素干预制备CAG大鼠模型。将成模大鼠按随机数字表法分为模型组、维酶素组及阴虚胃痛胶囊低剂量、中剂量、高剂量组。阴虚胃痛胶囊低剂量、中剂量、高剂量组分别给予205.2 mg/kg、410.4 mg/kg、820.8 mg/kg阴虚胃痛胶囊溶液灌胃,维酶素组给予270 mg/kg维酶素溶液灌胃,正常组与模型组给予等剂量生理盐水灌胃,均1次/d,共干预8周。苏木精-伊红(HE)染色观察大鼠胃黏膜病理情况;透射电镜观察大鼠胃黏膜超微结构变化;实时荧光定量聚合酶链反应检测胃组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的基因表达;蛋白质免疫法检测胃组织中炎症介质TNF-α、IL-1β和抗炎因子白细胞介素-4(IL-4)、白细胞介素-10(IL-10)的蛋白表达。结果:与正常组比较,模型组HE染色显示腺体减少;透射电镜显示壁细胞和主细胞结构被明显破坏,TNF-α、IL-1β的表达高(P<0.05),IL-4、IL-10表达低(P<0.05)。与模型组比较,阴虚胃痛胶囊中剂量、高剂量组及维酶素组HE提示腺体改善,透射电镜提示壁细胞和主细胞结构恢复,胃黏膜中TNF-α、IL-1β表达低(P<0.05),IL-4、IL-10表达高(P<0.05)。结论:阴虚胃痛胶囊可明显促进CAG大鼠胃黏膜异常超微结构的恢复,抑制炎症介质TNF-α、IL-1β表达,促进抗炎因子IL-4、IL-10表达。Objective:To observe the effects of Yinxu Weitong Capsules on the ultrastructure of gastric mucosa and inflammatory mediators in rats with chronic atrophic gastritis(CAG).Methods:A total of 72 specific pathogen-free(SPF) Sprague-Dawley(SD) rats were divided into a normal group(n=13) and an experimental group(n=59).The experimental group was given N-methyl-N′-nitro-N-nitrosoguanidine in drinking water and intragastric administration,combined with irregular feeding to induce a CAG rat model.The modeled rats were randomly divided into a model group,a vitacoenzyme group,and low-,medium-,and high-dose Yinxu Weitong Capsules groups.The Yinxu Weitong Capsules groups received intragastric administration of Yinxu Weitong Capsules at doses of 205.2 mg/kg,410.4 mg/kg,and 820.8 mg/kg,respectively.The vitacoenzyme group received vitacoenzyme solution at 270 mg/kg,and the normal and model groups received an equivalent dose of saline,all once daily for eight weeks.Hematoxylin-eosin(HE) staining was used to observe the pathological changes in the gastric mucosa.Transmission electron microscopy was used to observe ultrastructural changes.Real-time quantitative PCR was used to detect the gene expression of tumor necrosis factor-alpha(TNF-α) and interleukin-1 beta(IL-1β) in gastric tissue.Western blot was used to detect the protein expression of pro-inflammatory factors TNF-α and IL-1β and anti-inflammatory factors interleukin-4(IL-4) and interleukin-10(IL-10).Results:Compared with the normal group,HE staining in the model group showed reduced glandular tissue,and transmission electron microscopy showed significant structural damage to parietal and chief cells.Moreover,the expression of TNF-α and IL-1β was higher(P<0.05),and the expression of IL-4 and IL-10 was lower(P<0.05).Compared with the model group,the medium-and high-dose Yinxu Weitong Capsules groups and the vitacoenzyme group showed improved glandular tissue in HE staining,restored parietal and chief cell structures in transmission electron microscopy,lower express

关 键 词：阴虚胃痛胶囊 慢性萎缩性胃炎 超微结构 大鼠 肿瘤坏死因子-α 白细胞介素-1β 白细胞介素-4 白细胞介素-10 

分 类 号：R256.3[医药卫生—中医内科学]