电针刺激对脑出血大鼠运动功能及白质损伤的影响  

Effect of Electroacupuncture Stimulation on Motor Function and White Matter Injury in Rats with Intracerebral Hemorrhage

作　　者：游咏梅 郑玲 饶婷 陈白 林淑芳 黄赛娥 江一静 YOU Yongmei;ZHENG Ling;RAO Ting;CHEN Bai;LIN Shufang;HUANG Saie;JIANG Yijing(Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China;Fujian Province Key Laboratory of Rehabilitation Technology,Fuzhou 350003,China;Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学附属康复医院,福州350003 [2]福建省康复技术重点实验室,福州350003 [3]福建中医药大学,福州350122

出　　处：《世界中医药》2024年第14期2113-2118,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(82305356);福建省自然科学基金项目(2020J01762);福建省卫健委科技计划项目(2020QNA069,2021CXA040)。

摘　　要：目的:观察电针对脑出血(ICH)大鼠运动功能和白质损伤(WMI)的影响。方法:将36只无特定病原体(SPF)级大鼠按随机数字表法分为假手术(SHAM)组、ICH组、ICH+电针组,每组12只;其中ICH组、ICH+电针组被制备右侧纹状体脑出血模型;ICH+电针组给予电针曲池、足三里穴干预,30 min/次,1次/d,连续干预7 d;7 d后采用转角测试、转棒实验测试评估运动功能;劳克坚牢蓝(LFB)染色、髓鞘碱性蛋白(MBP)免疫荧光表达评估WMI情况;神经胶质细胞原纤维酸性蛋白(GFAP)和神经元核抗原(NeuN)免疫荧光染色检测星形胶质细胞活化和神经元损伤情况;酶联免疫吸附试验(ELISA)检测肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的表达水平。结果:电针治疗降低脑出血大鼠右转的频率、增加转棒停留时间(P<0.05),电针干预能够减少脑出血带来的髓鞘破坏、增加MBP表达、减少星形胶质细胞活化数量和神经元损伤、抑制TNF-α、IL-1β表达(P<0.05)。结论:电针通过降低了星形胶质细胞过度活化和神经炎症,减轻纹状体WMI和神经元损伤,促进ICH大鼠运动功能恢复。Objective:To observe the effects of electroacupuncture stimulation on motor function and white matter injury(WMI) in rats with intracerebral hemorrhage(ICH).Methods:A total of 36 specific pathogen-free(SPF) rats were randomly divided into sham operation(SHAM) group,ICH group,and ICH+electroacupuncture group,with 12 rats in each group.The ICH group and ICH+electroacupuncture group were used to prepare the right striatal ICH model.The ICH+electroacupuncture group was intervened in “Quchi” and “Zusanli” acupoints with electroacupuncture for 30 min each day for seven consecutive days.After seven days,the motor function was evaluated by the rotational angle test and the Rotarod test.Luxol fast blue(LFB) staining and myelin basic protein(MBP) immunofluorescence expression were used to evaluate WMI.Glial fibrillary acidic protein(GFAP) and neuronal nuclei(NeuN) immunofluorescence expressions were detected to assess the activation of astrocytes and the injury of neurons.The expression levels of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were detected by enzyme linked immunosorbent assay(ELISA).Results:Electroacupuncture treatment decreased the frequency of right turns and increased the retention time of rats with ICH in the Rotarod test(P<0.05).Electroacupuncture intervention could also reduce myelin destruction,increase the expression of MBP,alleviate activation of astrocytes and injury of neurons,and inhibit the expression of TNF-α and IL-1β(P<0.05).Conclusion:Electroacupuncture can reduce the overactivation of astrocytes and neuroinflammation,reduce WMI and injury of neurons in the striatum,and promote the recovery of motor function in rats with ICH.

关 键 词：电针 曲池 足三里 脑出血 运动功能 白质损伤 星形胶质细胞 神经炎症 

分 类 号：R245.97[医药卫生—针灸推拿学]