妊娠合并梅毒感染外周血miR-31表达与Th17/Treg相关细胞因子水平变化及临床意义  

Changes of miR-31,Th17/Treg-related cytokines in peripheral blood of pregnant women complicated with syphilis infection and their clinical significance

作　　者：李群 董福云 邢海燕[3] LI Qun;DONG Fu-yun;XING Hai-yan(Jinan Fourth People's Hospital,Jinan,Shandong 250000,China)

机构地区：[1]济南市第四人民医院产科,山东济南250000 [2]济南市第四人民医院妇科,山东济南250000 [3]济南市第四人民医院妇产科门诊,山东济南250000

出　　处：《中华医院感染学杂志》2024年第14期2197-2200,共4页Chinese Journal of Nosocomiology

基　　金：山东省重点研发计划基金资助项目(S2021-ZDYF-0368)。

摘　　要：目的 探讨妊娠合并梅毒感染患者外周血微小RNA-31(miR-31)表达与辅助性T淋巴细胞17/调节性T淋巴细胞(Th17/Treg)相关细胞因子水平变化及临床意义.方法 选取2019年6月-2023年6月济南市第四人民医院收治的106例妊娠合并梅毒感染孕妇为研究组,同期60名健康顺产孕妇为对照组,比较外周血Th17/Treg相关细胞因子水平及miR-31表达水平.研究组患者根据治疗预后分为正常转阴组73例、血清固定组33例,比较其外周血miR-31表达量、Th17/Treg相关细胞因子水平,采用受试者工作特征(ROC)曲线分析外周血miR-31表达量对妊娠合并梅毒感染孕妇治疗结局的预测价值.结果 研究组外周血miR-31、转化生长因子-β(TGF-β)及白细胞介素-10(IL-10)表达水平均低于对照组(P<0.05),而IL-17及TNF-α表达水平均高于对照组(P<0.05).正常转阴组外周血miR-31、TGF-β及IL-10表达水平均低于血清固定组(P<0.05),而IL-17及TNF-α表达水平均高于血清固定组(P<0.05);ROC曲线显示妊娠合并梅毒感染孕妇外周血miR-31表达水平预测治疗效果不良的最佳截断值为0.43,曲线下面积为0.788(95%CI:0.699~0.872),灵敏度、特异度分别为75.20%、70.33%.结论 妊娠合并梅毒感染孕妇外周血miR-31表达水平明显降低,且存在明显的Th17/Treg相关细胞因子水平失衡,对评估治疗效果有一定的价值.OBJECTIVE To explore the expression of micro ribonuclear acid-31(miR-31)and helper T cell 17/regu-latory T cell(Th17/Treg)-related cytokines in peripheral blood of the pregnant women complicated with syphilis infection and analyze the clinical significance.METHODS A total of 106 pregnant women complicated with syphilis infection who were treated in Jinan Fourth People's Hospital from Jun 2019 to Jun 2023 were assigned as the study group,meanwhile,60 healthy pregnant women who gave natural birth were chosen as the control group.The lev-els of peripheral blood Th17/Treg-related cytokines and expression level of miR-31 were observed and compared.The patients of the study group were divided into the normal negative conversion group with 73 cases and the ser-um fixed group with 33 cases according to the treatment outcomes,the expression level of peripheral blood miR-31 and levels of Th17/Treg-related cytokines were compared between the two groups.The value of the peripheral blood miR-31 in prediction of the treatment outcomes of the pregnant women complicated with syphilis infection was analyzed by means of receiver operating characteristic(ROC)curves.RESULTS The expression levels of pe-ripheral blood miR-31,transforming growth factor-β(TGF-β)and interleukin-10(IL-10)of the study group were significantly lower than those of the control group(P<0.05),while the expression levels of IL-17 and TNF-αof the study group were significantly higher than those of the control group(P<0.05).The expression levels of pe-ripheral blood miR-31,TGF-βand IL-10 of the normal negative conversion group were significantly lower than those of the serum fixed group(P<0.05),while the expression levels of IL-17 and TNF-αof the normal negative conversion group were higher than those of the serum fixed group(P<0.05).ROC curve analysis showed that the optimal cutoff value of the peripheral blood miR-31 was 0.43 in prediction of poor curative effect on the pregnant women complicated with syphilis infection,the area under curve was 0.788(95%CI:

关 键 词：妊娠合并梅毒感染 微小RNA-31 辅助性T淋巴细胞17/调节性T淋巴细胞相关细胞因子 治疗效果 预测价值 

分 类 号：R714.259[医药卫生—妇产科学]