七氟烷对胶质瘤U87细胞侵袭能力的影响及机制研究  

Effect of Sevoflurane on Invasion Ability of Glioma U87 Cells and Its Mechanism

作　　者：郝景茹[1] 宋雨桐 高灿[1] HAO Jingru;SONG Yutong;GAO Can(NMPA Key Laboratory for Research and Evaluation of Narcotic and Psychotropic Drugs,Jiangsu Province Key Laboratory of Anesthesiology,Jiangsu Province Key Laboratory of Anesthesia and Analgesia Application,Xuzhou Medical University,Xuzhou,Jiangsu Province,221004 China)

机构地区：[1]国家药品监督管理局麻醉药品和精神药品研究与评价重点实验室,江苏省麻醉学重点实验室,江苏省麻醉与镇痛应用重点实验室,徐州医科大学,江苏徐州221004

出　　处：《系统医学》2024年第11期1-4,共4页Systems Medicine

基　　金：国家自然科学基金(82371215)。

摘　　要：目的探讨七氟烷对U87细胞侵袭能力的影响及机制。方法于2022年9月—2023年6月,采用徐州医科大学麻醉学实验室提供的U87细胞培养至对数生长期。Transwell实验取对数生长期细胞分成8份,然后随机分为对照(Con)组和七氟烷(Sev)组,每组4份进行平行实验。培养的细胞置于麻醉箱内,七氟烷组通载体气体和1.4%七氟烷4 h,对照组只通载体气体,观察七氟烷对U87细胞侵袭能力的影响;免疫印迹实验取对数生长期细胞分成12份,然后随机分为对照组,七氟烷2 h组和七氟烷4 h组,每组4份进行平行实验,观察七氟烷对pERK1/2、ERK1/2表达水平的影响;细胞免疫荧光化学实验取对数生长期细胞分成8份,然后随机分为对照组和七氟烷组(4 h),每组4份进行平行实验,检测七氟烷对pERK1/2、Nrf2在细胞分布的影响。结果与对照组相比,七氟烷组U87细胞穿过微孔膜的数目减少[Con组(100.000±0.000)%,Sev组(34.180±3.342)%],差异有统计学意义(t=16.697,P<0.05)。与对照组相比,七氟烷组pERK1/2的灰度值下降[Con组(100.000±0.000)%,Sev2 h组(0.196±0.028)%,Sev4 h组(0.073±0.025)%],差异有统计学意义(F=546.886,P<0.05),且核内pERK1/2[Con组(21.903±1.776)%,Sev组(8.412±0.367)%]及核内Nrf2表达水平均显著下降[Con组(18.083±0.788)%,Sev组(12.315±1.604)%],差异有统计学意义(t=7.438、3.228,P均<0.05)。结论七氟烷通过抑制ERK1/2-Nrf2信号通路抑制人胶质细胞瘤U87侵袭能力。Objective To explore the impact of sevoflurane on U87 cells migration.Methods U87 cells,which pro-vided by anesthesiology Laboratory of Xuzhou Medical University were cultured to logarithmic growth stage and then randomly random grouping for all experiments.In Transwell experiment,cells were divided into 8 parts,and then ran-domly divided into control(Con)and sevoflurane(Sev)groups(with 4 parts in each group for parallel experiments)to detect the impact of sevoflurane on the migration between September 2022 and June 2023.Cultured cells were placed in an anaesthetic chamber 1.4%sevoflurane with carrier gas in the sevoflurane group and carrier gas only in the con-trol group for 4 h.The Transwel assay was used to evaluate the migration of U87 cells;In WB experiment,cells were divided into 12 parts,and then randomly divided into control group,sevoflurane 2 h group and sevoflurane 4 h group(with 4 parts in each group for parallel experiments)to evaluate the level of pERK1/2 and ERK1/2 expression;In im-munofluorescence experiment,cells at logarithmic growth stage were divided into 8 parts,and then randomly divided into control and sevoflurane groups(4 h)(with 4 parts in each group for parallel experiments)to detect pERK1/2 and Nrf2 cell distribution.Results Compared with the control group,the number of U87 cells passing through the micropo-rous membrane in sevoflurane group decreased[Con group(100.000±0.000)%,Sev group(34.180±3.342)%],and the difference was statistically significant(t=16.697,P<0.05).Compared with the control group,the gray value of pERK1/2 in sevoflurane group decreased[Con group(100.000±0.000)%,Sev2 h group(0.196±0.028)%,Sev4 h group(0.073±0.025)%],and the difference was statistically significant(F=546.886,P<0.05).The expression levels of pERK1/2[Con group(21.903±1.776)%,Sev group(8.412±0.367)%]and Nrf2 were significantly decreased[Con group(18.083±0.788)%,Sev group(12.315±1.604)%],the differences were statistically significant(t=7.438,3.228,both P<0.05).Conclusion Sevoflurane inhibits the in

关 键 词：七氟烷 细胞外信号调节激酶 转录因子NF-E2相关因子2 人胶质细胞瘤 

分 类 号：R34[医药卫生—基础医学]