人源性免疫重塑免疫缺陷小鼠模型的构建  

Construction of human immune remodeling immunodeficient mouse model

作　　者：孙焕友 吕建成 杨潇[1] 于浩[1] 白珂欣 庄俊涛 吕强[1] Sun Huanyou;Lyu Jiancheng;Yang Xiao;Yu Hao;Bai Kexin;Zhuang Juntao;Lyu Qiang(Department of Urology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院泌尿外科,南京210029

出　　处：《中华实验外科杂志》2024年第6期1139-1142,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(82273152、82072832);江苏省人民医院临床能力提升项目(规范)(JSPH-MA-2022-5)。

摘　　要：目的探讨通过尾静脉回输人源外周血单个核细胞来构建人源性免疫重塑小鼠模型的可能性。方法招募了5位供血者,从5位供血者肘正中静脉抽取20 ml静脉血,分别从抽取血液中提取外周血单核细胞(PBMC),计数完成后用150μl磷酸盐缓冲液(PBS)重悬1×10^(7)PBMC,然后分别对5组(每位供血者分别对应一组小鼠,各组再根据3种不同注射浓度分为3个亚组,注射浓度依次为1×10^(6)/只、1×10^(7)/只、1×10^(8)/只)超重度免疫缺陷(NOG)小鼠(6周龄)进行尾静脉回输。1周后从小鼠眼眶中采集血液样本,使用人源白细胞共同抗原45(CD45)抗体标记染色,应用流式细胞术检测人源CD45阳性PBMC细胞的比例,采用One-Way ANOVA方差分析。结果在回输数量级为1×10^(7)/只的NOG小鼠外周血中的人源CD45+PBMC细胞比例最高,可达70.0%以上,其中一号供血者的第2组小鼠外周血中的人源CD45+PBMC细胞比例高于一号供血者的第1组小鼠[(73.17±1.73)%比(40.23±1.71)%,F=483.982,P<0.01],但和第3组小鼠无显著差异[(73.17±1.73)%比(69.18±2.07)%,F=1.630,P>0.05]。此外,一号供血者的第2组小鼠平均不良反应出现时间长于第3组小鼠[(28±0)d比(21±2)d,F=36.750,P<0.01]。结论通过对免疫缺陷NOG小鼠进行尾静脉回输人源外周血单个核细胞的方式,可以有效的构建稳定的人源免疫重塑小鼠模型。Objective To explore the feasibility of constructing a humanized immune reconstituted mouse model by reinfusing human peripheral blood mononuclear cells(PBMCs)via the tail vein.Methods We recruited 5 blood donors and drew 20 ml venous blood from the median cubital vein.Then we obtained human PBMCs from the peripheral venous blood.NOG mice(obtained from Beijing Vital River Laboratory Animal Technology Co.,Ltd.,China)were divided into 5 groups(Each blood donor corresponds to a group of mice,and each group was divided into three subgroups according to three different injection concentrations,respectively 1×10^(6)/mouse,1×10^(7)/mouse,and 1×10^(8)/mouse).One week later,blood samples were collected from the orbital plexus of the mice,stained with human CD45 antibody(Biolegend,USA),and the proportion of human CD45+PBMC cells was detected by flow cytometry,followed by t-test analysis.Results The proportion of human CD45+PBMCs in the peripheral blood of NOG mice that received 1×10^(7)cells per mouse reached over 70.0%.In donor group one,the proportion of human CD45+PBMC cells in peripheral blood of mice in the second group was higher than that of mice in the first group[(73.17±1.73)%vs.(40.23±1.71)%,F=483.982,P<0.01],but not significantly different from that in the third group[(73.17±1.73)%vs.(69.18±2.07)%,F=1.630,P>0.05].Additionally,the average duration of adverse reactions of mice in the second group was longer than that in the third group[(28±0)d vs.(21±2)d,F=36.750,P<0.01].These results indicate the successful establishment of a stable humanized immune reconstituted NOG mouse model.Conclusion A stable humanized immune reconstituted mouse model can be effectively established by reinfusing human PBMCs into immunodeficient NOG mice via the caudal vein.

关 键 词：免疫回输 外周血 单核细胞 免疫缺陷 

分 类 号：R392[医药卫生—免疫学] R-332[医药卫生—基础医学]