乙醛酸诱导肥胖小鼠肾草酸盐结石的模型建立  

Establishment of renal oxalate stone model in obese mice induced by glyoxalic acid

作　　者：吴鹏飞 刘宇超 杨扶涵 张文涛 张竞成 姚旭东 Wu Pengfei;Liu Yuchao;Yang Fuhan;Zhang Wentao;Zhang Jingcheng;Yao Xudong(Department of Urology,Shanghai tenth School of Clinical Medicine,Nanjing Medical University,Shanghai Tenth People's Hospital,Shanghai 200072,China)

机构地区：[1]南京医科大学上海十院临床医学院,上海市第十人民医院泌尿外科,上海200072

出　　处：《中华实验外科杂志》2024年第6期1177-1180,共4页Chinese Journal of Experimental Surgery

基　　金：上海市科学技术委员会基金资助项目(22140903800)。

摘　　要：目的探讨乙醛酸诱导下肥胖小鼠肾草酸盐结石形成的模型建立与验证。方法选6周龄健康的无特定病原体(SPF)的C57BL/6N雄鼠24只,经过中国医学科学实验动物研究所质量检测,无特定病原体级,饲养于上海市第十人民医院科创中心实验室;普通饲料统一喂养1周。小鼠采用随机数字表法分为两组:对照组12只,采取普通饲料喂养;高脂实验组12只,采用高脂饲料喂养18周,诱导小鼠肥胖模型。将对照组12只小鼠采用随机数字表法分为腹腔注射乙醛酸的乙醛酸组6只和PBS组(腹腔注射磷酸盐缓冲液)6只。后将高脂实验同样随机分为肥胖乙醛酸组6只和肥胖组6只。乙醛酸组小鼠腹腔注射乙醛酸溶液连续7 d后检测4组小鼠血浆及肾脏中检测的草酸盐含量,比较4组小鼠草酸盐含量变化。两组间比较采用配对t检验,符合正态分布的计量资料以均数±标准差(±s)表示。结果4组小鼠的初始体重由(21.45±2.54)g到对照组(36.42±2.38)g,高脂实验组(44.91±2.89)g,体重相差大于20%。PBS组、肥胖组、乙醛酸组、肥胖乙醛酸组小鼠血浆中的草酸盐含量比较,差异有统计学意义[PBS组比乙醛酸组:(57.18±39.60)μmol/L比(281.11±33.54)μmol/L;PBS组比肥胖组:(57.18±39.60)μmol/L比(529.87±241.61)μmol/L;肥胖组比肥胖乙醛酸组:(529.87±241.61)μmol/L比(1156.46±186.83)μmol/L;乙醛酸组比肥胖乙醛酸组:(281.11±33.54)μmol/L比(1156.46±186.83)μmol/L,t=13.62、5.87、5.41、11.46,P<0.01]。PBS组、肥胖组、乙醛酸组、肥胖乙醛酸组小鼠肾脏中草酸盐含量比较,差异有统计学意义[PBS组比乙醛酸组:(377.96±148.39)μmol/L比(805.28±239.49)μmol/L;PBS组比肥胖组:(377.96±148.39)μmol/L比(1386.19±310.36)μmol/L;肥胖组比肥胖乙醛酸组:(1386.19±310.36)μmol/L比(3810.19±477.73)μmol/L;乙醛酸组比肥胖乙醛酸组:(805.28±239.49)μmol/L比(3810.19±477.73)μmol/L,t=5.57、6.63、11.46、14.54,P<0.01]。结论肥胖以及腹�Objective To establish and verify the model of kidney oxalate stone formation induced by glyoxylic acid in obese mice.Methods A total of 246-week-old healthy C57BL/6N male mice without specific pathogen(SPF)were selected and kept in the laboratory of Science Innovation Center of Shanghai 10th People’s Hospital after quality inspection by China Institute of Medical Laboratory Animals.Common diet was fed uniformly for 1 week.The mice were divided into two groups by a random number table method:control group(12 mice were fed on ordinary diet);high-fat experimental group(12 mice were fed on high-fat diet for 18 weeks to induce obesity model).The control group of 12 were randomly divided into 6 glyoxylic acid group and 6 PBS group(intraperitoneal injection of phosphate buffer salt solution).Then the high-fat experimental group was randomly divided into obese glyoxylate group 6 and obese group 6.The oxalate content in plasma and kidney of mice in the glyoxylic acid group was determined after intraperitoneal injection of glyoxylic acid solution for 7 consecutive days,and the changes in oxalate content in the 4 groups were compared.A paired t test was used for comparison between the two groups.Measurements conforming to the normal distribution were expressed as mean±standard deviation(±s).Results The initial body weight of mice in the 4 groups was from(21.45±2.54)g to(36.42±2.38)g in the control group and(44.91±2.89)g in the high-fat experimental group,with a difference of more than 20%.The average plasma oxalate content in PBS group,obesity group,glyoxylate group and obesity glyoxylate group was compared[PBS group(57.18±39.60)vs.glyoxylate group(281.11±33.54)μmol/L,t=13.62,P<0.01;PBS group(57.18±39.60)vs.obese group(529.87±241.61)μmol/L,t=5.87,P<0.01;obesity group(529.87±241.61)vs.obese glyoxylate group(1156.46±186.83)μmol/L,t=5.41,P<0.01;glyoxylate group(281.11±33.54)vs.obese glyoxylate group(1156.46±186.83)μmol/L,t=11.46,P<0.01].Similarly,the average oxalate content in kidney among all groups was(37

关 键 词：肾结石 肥胖 草酸盐 模型 动物 

分 类 号：R692.4[医药卫生—泌尿科学]