可见光增强的MnO_(x)@g-C_(3)N_(4)纳米酶用于检测亚硫酸根  

Visible light-enhanced MnO_(x)@g-C_(3)N_(4)nanozymes for the detection of sulfite ions

作　　者：史亚妮 原源 张冰 赵志换 林建英 SHI Yani;YUAN Yuan;ZHANG Bing;ZHAO Zhihuan;LIN Jianying(College of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China)

机构地区：[1]太原理工大学生物医学工程学院,太原030024

出　　处：《分析试验室》2024年第7期991-997,共7页Chinese Journal of Analysis Laboratory

基　　金：山西省应用基础研究计划面上项目(20210302123142)资助。

摘　　要：基于可见光增强的Mn基纳米酶,开发出一种检测亚硫酸根(SO_(3)^(2-))的比色传感器。通过简单的室温沉淀法合成MnO_(x)@g-C_(3)N_(4)样品,其具有类氧化酶活性和光催化活性。在可见光照射下,它的类氧化酶活性增强,可以催化氧化显色底物3,3',5,5'-四甲基联苯胺(TMB)为蓝色氧化物oxTMB。其米氏常数Km为0.059 mmol/L,最大反应速率Vmax为0.53×10^(-9)(mol/L)/s。基于SO_(3)^(2-)对MnOx@g-C_(3)N_(4)/TMB体系的抑制作用,最佳条件下该方法检测SO_(3)^(2-)的线性范围为2.5~30μmol/L,检出限为2.2μmol/L。A colorimetric sensor for the detection of sulfite ions(SO_(3)^(2-))was developed based on Mn-based nanoenzyme regulated by visible light.The MnO_(x)@g-C_(3)N_(4)sample with oxidase-like activity and photocatalytic activity was synthesized by a simple precipitation method at room temperature.Under visible light irradiation,its oxidase-like activity could be enhanced to catalyze the oxidation of chromogenic substrate 3,3',5,5'-tetramethylbenzidine(TMB)to blue oxTMB.The Michaelis-Menten constant K_(m) was 0.059 mmol/L,and the maximum reaction rate V_(max)was 0.53×10^(-9)(mol/L)/s.Based on the inhibition effect of SO_(3)^(2-)on MnO_x@g-C_(3)N_(4)/TMB system,the linear range of SO_(3)^(2-)was 2.5-30μmol/L and the limit of detection was 2.2μmol/L under the optimal conditions.

关 键 词：比色传感器 光催化 氧化酶活性 MnOx@g-C_(3)N_(4) 

分 类 号：O652.7[理学—分析化学]