反相高效液相色谱法同时拆分3种手性杀菌剂  

Simultaneous enantiomeric separation of three triazole fungicides by reversed-phase high performance liquid chromatography

作　　者：周励 唐婧 范荣华 陈彤 欧阳玉 ZHOU Li;TANG Jing;FAN Ronghua;CHEN Tong;OUYANG Yu(School of Public Health,Shenyang Medical College,Shenyang 110034,China;School of Clinical Medicine,Beijing Tsinghua Changgung Hospital,Tsinghua University,Beijing 102218,China)

机构地区：[1]沈阳医学院公共卫生学院,沈阳110034 [2]清华大学临床医学院,清华大学附属北京清华长庚医院,北京102218

出　　处：《分析试验室》2024年第6期774-779,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(82204343);辽宁省博士科研启动基金计划项目(2022-BS-343)资助。

摘　　要：应用Chiralpak IH手性色谱柱,建立了一种同时拆分多效唑、己唑醇、糠菌唑3种三唑类杀菌剂对映异构体的高效液相色谱分析方法。采用反相色谱条件,以乙腈-水为流动相,在230 nm下进行分析,在优化条件下,即乙腈-水(40∶60, V/V)为流动相,柱温35℃、流速1.0 mL/min时,3种三唑类杀菌剂对映异构体能同时实现手性分离。A high performance liquid chromatographic method was established for the simultaneous chiral separation of three triazole fungicides,including paclobutrazol,hexaconazole and bromuconazole,using Chiralpak IH column.The analysis of three triazole fungicides was carried out by reversed-phase high performance liquid chromatography with the mobile phase of acetonitrile and water.The wavelength of UV detector was 230 nm.Under the optimized separation conditions as follows:acetonitrile-water(40:60,V/V)as mobile phase,column temperature of 35℃and flow rate of 1.0 mL/min,three triazole fungicides were chiral separated simultaneously.

关 键 词：高效液相色谱法 手性分离 反相 Chiralpak IH手性柱 三唑类杀菌剂 

分 类 号：TQ450.7[化学工程—农药化工]