碳点比率荧光探针测定果蔬中的甲胺磷  

Carbon dots-based ratiometric fluorescent probe for the detection of methamidophos residues in fruits and vegetables

作　　者：韩雍[1] 汪慧[1] 张晓燕 HAN Yong;WANG Hui;ZHANG Xiaoyan(College of Agriculture and Forestry,Longdong University,Qingyang 745000,China)

机构地区：[1]陇东学院农林科技学院,庆阳745000

出　　处：《分析试验室》2024年第6期780-784,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(32060571);甘肃省科技项目(21JR7RM186,22JR5RM204,23CXGM0001,QY2021A-F017);中国科学院“西部之光”人才基金(2020XBZGXBQNXZ-A)资助。

摘　　要：以邻苯二胺与浓硫酸为原料,通过一步水热法合成了荧光碳点(oPD-CDs),构建了一种检测果蔬中甲胺磷(MTP)的oPD-CDs比率荧光传感器。在优化条件下,oPD-CDs传感器的荧光比值(F_(430)/F_(483))与甲胺磷浓度在0~160 ng/mL范围内呈良好的线性关系,相关系数R^(2)为0.99,检出限(LOD)为14.3 ng/mL,加标回收率为92.0%~108.0%,相对标准偏差(RSDs)为0.7%~1.6%。该方法可用于果蔬中甲胺磷残留的检测。In this work,o-phenylenediamine and concentrated sulfuric acid were used as precursors to prepare multi-emissive fluorescent carbon dots(oPD-CDs)through a one-step hydrothermal method.Then,oPD-CDs were employed as a fluorescent probe for the detection of methamidophos(MTP)residues in fruits and vegetables.Under the optimized conditions,the fluorescence ratio values(F_(430)/F_(483))of the oPD-CDs sensor showed a good linear relationship with the MTP concentration in the range of 0-160 ng/mL with the correlation coefficient(R^(2))of 0.99.The limit of detection(LOD)was calculated as 14.3 ng/mL,the spiked recoveries were 92.0%-108.0%with the relative standard deviations(RSDs)of 0.7%-1.6%.The method can be applied to the detection of MTP residues in fruits and vegetables.

关 键 词：农药残留 荧光碳点 甲胺磷 快速检测 

分 类 号：O657.3[理学—分析化学] O661.1[理学—化学]