香豆素衍生物-纸芯片体系对3种生物硫醇的选择性检测  

Selective detection of three biomercaptan by a coumarin derivative based paper chip

作　　者：张剑 王祖黎 唐新萍 李一冰 郑志鸿 王月萍 张博[1] 刘春叶[1] ZHANG Jian;WANG Zuli;TANG Xinping;LI Yibing;ZHENG Zhihong;WANG Yueping;ZHANG Bo;LIU Chunye(School of Pharmacy,Xi'an Medical University,Xi'an 710021,China)

机构地区：[1]西安医学院药学院,西安710021

出　　处：《分析试验室》2024年第6期814-818,共5页Chinese Journal of Analysis Laboratory

基　　金：陕西省科技厅项目(2023-YBSF-202);西安医学院产业化培育项目(21CYH01);西安医学院科研能力提升计划项目(2022NLTS063,2022NLTS069);大学生开放实验科研基金项目(2021DXS58,2021DXS68)资助。

摘　　要：合成了一种7-(二乙氨基)香豆素衍生物荧光探针,并通过红外吸收光谱、质谱和核磁共振波谱对其进行结构表征。该探针可与同型半胱氨酸(Hcy)、半胱氨酸(Cys)、谷胱甘肽(GSH)发生不同的显色反应,以微流控纸芯片为平台,实现了对3种生物硫醇的特异性检测。手机拍照记录显色结果,Photpshop软件分析RGB通道颜色强度(RGB值)。当探针浓度为10μmol/L, 3种生物硫醇浓度在10~50μmol/L范围内时,RGB值与生物硫醇浓度呈良好线性关系。该方法在6 min内即可完成实际样品分析,结果相对误差均小于3%,可用于疾病危险因子的快速实时检测。A fluorescent probe of 7-(diethylamino)coumarin derivative was synthesized,and characterized by fourier transform infrared(FT-IR)spectroscopy,mass spectrometry(MS)and nuclear magnetic resonance(NMR)spectroscopy,respectively.This probe is capable of interacting with homocysteine(Hcy),cysteine(Cys),and glutathione(GSH)and present different colors,which supports a distinguishing detection of three biomercaptan using microfluidic paper chips as a platform.The result photos were recorded using a mobile phone,and the color intensity of RGB channel(RGB value)were assessed by Photoshop software.Favourable linear relationships were obtained between color intensity and concentrations of the three biomercaptan in the range of 10-50μmol/L with the probe concentration of 10μmol/L.The real sample was analyzed within 6 min,and the relative errors of the results were all less than 3%.The work demonstrates that the method is enable to be employed for rapid and real-time detection of risk factors of diseases.

关 键 词：荧光探针 微流控纸芯片 同型半胱氨酸 半胱氨酸 谷胱甘肽 

分 类 号：O652[理学—分析化学]