合成大麻素4CN-BINACA-ADB斑马鱼体内代谢研究  

Study on metabolites of synthetic cannabinoid 4CN-BINACAADB in zebrafish

作　　者：谢宜陈 茹俊辉 周善慧 王旻昊 吴剑丙 范一雷 XIE Yichen;RU Junhui;ZHOU Shanhui;WANG Minhao;WU Jianbing;FAN Yilei(Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province,Zhejiang Police College,Hangzhou 310052,China)

机构地区：[1]浙江警察学院,浙江省毒品防控技术研究重点实验室,杭州310052

出　　处：《分析试验室》2024年第6期892-899,共8页Chinese Journal of Analysis Laboratory

基　　金：浙江省基础公益研究计划项目(LTGC23B050003);浙江省毒品防控技术研究重点实验室开放课题(202006);2022年国家级大学生创新创业训练计划项目(202211483025)资助。

摘　　要：采用斑马鱼模型结合超高效液相色谱串联四极杆-静电场轨迹离子阱Q Exactive质谱(UHPLC-QObitrap-HRMS)技术,研究合成大麻素4CN-BINACA-ADB的体内代谢途径。结果表明,4CN-BINACAADB共产生酯水解、羧酸化、羟基化、氰基被羟基还原取代、N-烷基化等Ⅰ相代谢物11种,葡萄糖醛酸化、硫酸化等Ⅱ相代谢物3种。结合代谢物的独特性和含量,推荐羧酸化产物C_(20)H_(24)N_(4)O_(5)(M2.1)以及羧酸化和氰基还原产物C_(20)H_(26)N_(4)O_(5)(M2.2)作为主要代谢标志物。研究结果可为生物样品中4CN-BINACAADB代谢物的检验鉴定提供理论支撑。The metabolic pathway of the synthetic cannabinoid 4CN-BINACA-ADB was investigated using ultra-high performance liquid chromatography coupled quadrupole-Orbitrap high-resolution mass spectrometry(UHPLC-Q-Obitrap-HRMS)in the zebrafish model.The results showed that 4CN-BINACA-ADB produced a total of eleven phase I metabolites from ester hydrolysis,carboxylation,N-alkylation,hydroxylation,and reduction of cyanide by hydroxyl,and three phase II metabolites from glucuronidation and sulfation.Carboxylation products C_(20)H_(24)N_(4)O_(5)(M2.1)and C_(20)H_(26)N_(4)O_(5)(M2.2)may be used as the main metabolic biomarkers of 4CN-BINACAADB in vivo.This study provides theoretical support for inspection appraisal of 4CN-BINACAADB metabolites in biological samples.

关 键 词：4CN-BINACA-ADB 合成大麻素 斑马鱼 代谢产物 代谢途径 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]