C57BL/6小鼠微小三毛滴虫的观察与种系发育分析  

Observation and phylogenetic analysis of Tritrichomonas minuta in C57BL/6 mice

作　　者：温福利[1] Wen Fuli(Shengli Clinical Medical College of Fujian Medical University/Fujian Provincial Hospital/Fuzhou University Affiliated Provincial Hospital,Fuzhou 350001)

机构地区：[1]福建医科大学省立临床医学院/福建省立医院/福州大学附属省立医院,福州350001

出　　处：《福建畜牧兽医》2024年第4期10-12,18,共4页Fujian Journal of Animal Husbandry and Veterinary medicine

摘　　要：为鉴定从某外单位引进的C57BL/6小鼠粪样检测中发现的虫体种类,本试验对虫体进行形态学观察,并进行种系发育分析。取C57BL/6小鼠粪样涂片和瑞士染色后进行显微镜观察,提取粪样总DNA,根据三毛滴虫的16S rRNA进行PCR扩增后测序,运用MEGA11进行种系发育分析。显微镜观察发现,虫体符合微小三毛滴虫的形态学特征,测序发现微小三毛滴虫的基因序列与鼠三毛滴虫高度同源。In order to identify the species of parasites found in the fecal samples of C57BL/6 mice introduced from a unit,the morphology of parasites was observed and the phylogenetic analysis was carried out.The fecal smears of C57BL/6 mice were stained with Swiss stain and observed under microscope.The total DNA of fecal samples was extracted,the 16S rRNA of Tritrichomonas murism was amplified by PCR and sequenced,Mega11 was used for phylogenetic analysis.Microscopical observation showed that the parasite conformed to the morphological characteristics of Tritrichomonas minuta,and sequencing showed that the gene sequence of Tritrichomonas minuta was highly homologous with that of Tritrichomonas murism.

关 键 词：C57BL/6小鼠 微小三毛滴虫 16S rRNA 种系发育分析 

分 类 号：S852.72[农业科学—基础兽医学]