^(60)Co-γ辐照处理对冬瓜种子影响初探  

Effect of ^(60)Co-γRadiation on Seeds of Wax Gourd

作　　者：弭宝彬[1] 刘丹 徐远芳 肖伟 谢玲玲[1] MI Bao-bin;LIU Dan;XU Yuan-fang;XIAO Wei;XIE Ling-ling(Hunan Vegetable Research Institute,Changsha 410125,PRC;Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding,Changsha 410125,PRC)

机构地区：[1]湖南省蔬菜研究所,湖南长沙410125 [2]湖南省核农学与航天育种研究所,湖南长沙410125

出　　处：《湖南农业科学》2024年第7期1-6,共6页Hunan Agricultural Sciences

基　　金：湖南省创新型省份建设专项经费(2021NK1006);湖南省科技人才支持项目(2022TJ-N15);湖南省自然科学基金(2022JJ30348)。

摘　　要：为丰富冬瓜种质资源,研究利用0、300、^(60)0、900 Gy剂量的^(60)Co-γ射线对冬瓜干种子和提前浸泡12 h的湿种子进行辐射诱变,处理后进行催芽及播种,考察辐照处理对种子萌发及幼苗光合性能、抗氧化酶活等的影响。结果表明:中低辐射剂量可以促进干、湿种子的早期萌发,且300 Gy辐射剂量的促进效果最佳;辐照处理对种子芽的伸长和不定根的发育呈现出明显的抑制效果;辐照处理会导致冬瓜幼苗真叶出现畸形,且其叶片细胞结构、光合参数、过氧化物酶酶活和丙二醛含量与未经辐照处理的叶片差异显著。该研究初步获得了冬瓜辐照处理的可行剂量和诱变子代,为后续冬瓜的辐射诱变剂量选择提供参考。To broaden the germplasm resources of wax gourd,we created new germplasm using ^(60)Co-γirradiation.The dry seeds and the seeds soaked for 12 h were irradiated with ^(60)Co-γat 0,300,^(60)0,and 900 Gy,respectively.The germinated seeds were sown,and the effects of irradiation on the germination of seeds and the photosynthetic performance and antioxidant enzyme activities of the seedlings were investigated.The results showed that low-and medium-dose irradiation promoted seed germination,and the irradiation at 300 Gy demonstrated the strongest promoting effect.Irradiation inhibited the bud growth and adventive root development.Irradiation treatments induced leaf deformity of the seedlings.Moreover,the leaf cell structure,photosynthetic parameters,peroxidase activity,and malondiadehyde content showed significant differences between irradiation treatments and the control.This study determined the optimal dose of irradiation for inducing mutagenesis and obtained the M1 generation,providing a reference for subsequent large-scale mutagenesis of wax gourd.

关 键 词：冬瓜种子 辐射诱变 种质资源 性状调查 

分 类 号：S642.3[农业科学—蔬菜学]