曼氏无针乌贼(Sepiella japonica)在不同盐度胁迫下的生理生化和组织学响应特征分析  

CHARACTERIZATION OF PHYSIOLOGICAL,BIOCHEMICAL,AND HISTOLOGICAL RESPONSES OF SEPIELLA JAPONICA UNDER SALINITY STRESS

作　　者：郝晴 崔闻博 谷敏 李双 周旭 迟长凤 HAO Qing;CUI Wen-Bo;GU Min;LI Shuang;ZHOU Xu;CHI Chang-Feng(National and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization,School of Marine Science and Technology,Zhejiang Ocean University,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学海洋科学与技术学院、海洋生物种质资源发掘利用国家地方联合工程研究中心,浙江舟山316022

出　　处：《海洋与湖沼》2024年第4期951-959,共9页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金项目,31872547号;浙江省自然科学基金项目,LTGN24C190005号。

摘　　要：盐度是影响海洋生物代谢和渗透调节最重要的生态因子之一,它的变化会影响头足类动物的能量收支、胚胎发育、个体生长、免疫能力、繁殖能力等多个方面,还会直接影响血清渗透压、离子浓度、抗氧化能力和游离氨基酸含量。为探究盐度胁迫对成体曼氏无针乌贼(Sepiella japonica)生理生化和组织学的影响,分别利用高盐(32)和低盐(18)对乌贼进行胁迫处理。结果显示,低盐胁迫6、12和24 h后,乌贼血淋巴中的Na^(+)、Cl^(-)、K^(+)、Ca^(2+)浓度相较于正常盐度组均显著性下降。高盐胁迫12和24 h后,血淋巴中的Na+浓度相较于正常盐度组显著性上升;Ca^(2+)浓度在12 h后显著性上升。肝脏中SOD活力和CAT的表达量在盐度胁迫6 h后显著下降。鳃中Na^(+)/K^(+)-ATP酶α亚基和Ca^(2+)-ATP酶的表达量在低盐胁迫24 h后显著下降。此外,H.E染色显示,低盐胁迫会造成乌贼鳃和肾组织核质比降低以及肝脏的病理性损伤,而在高盐胁迫后无变化。研究结果从生理生化水平和组织学水平解析曼氏无针乌贼成体在不同盐度胁迫下的响应特征,为曼氏无针乌贼和头足类的盐度响应研究提供了形态学支撑和理论依据,也对乌贼养殖过程中应对盐度波动具有一定的参考价值。Salinity is a key ecological factor affecting the metabolism and osmoregulation of marine organisms.Changes in salinity can affect energy expenditure,embryonic development,individual growth,immunity,reproduction,serum osmolality,ionic concentration,antioxidant capacity,and free amino acid content in cephalopods.To investigate the effects of salinity stress on Sepiella japonica,adult cuttlefish were subjected to high(32)and low salinity(18)conditions.Results showed that the concentrations of Na^(+),Cl^(-),K^(+),and Ca^(2+)in the hemolymph of the cuttlefish decreased significantly under 6,12,and 24 hours of low salinity stress exposure.Additionally,the Na+concentration in the hemolymph increased significantly after 12 and 24 hours of high salinity stress treatment,while the Ca^(2+)concentration increased significantly after 12 hours treatment.A significant decrease in SOD activity and CAT expression was observed in the liver after 6 hours salinity stress exposure.After 24 hours low salinity stress treatment,the mRNA expression level of Na^(+)/K^(+)-ATPaseα-subunit and Ca^(2+)-ATPase in the gills significantly decreased.Moreover,the H&E staining revealed a decrease in the nucleocytoplasmic ratios of the cuttlefish gill and kidney tissues,and pathological damage to the liver,which remained unchanged after high salinity stress exposure.The findings provide a morphological support and a theoretical basis for studying the salinity response of S.japonica and cephalopods,and further have practical implications for dealing with salinity mutations in cuttlefish culture.

关 键 词：曼氏无针乌贼 盐度胁迫 生理生化特征 酶活性 组织学分析 

分 类 号：S931[农业科学—渔业资源] S968[农业科学—水产科学] Q955[生物学—动物学]