舟形藻生物膜诱导下虾夷扇贝(Mizuhopecten yessoensis)幼虫的变态及其蛋白质组学响应  

METAMORPHOSIS OF MIZUHOPECTEN YESSOENSIS LARVAE INDUCED BY NAVICULA sp.AND ITS PROTEOMIC RESPONSE

作　　者：徐筱琰 杜美荣[2,3,4] 蒋增杰 姜娓娓[2,3,4] 夏苏东 秦晓芳[2,3,4] 严瑾 吕建福 XU Xiao-Yan;DU Mei-Rong;JIANG Zeng-Jie;JIANG Wei-Wei;XIA Su-Dong;QIN Xiao-Fang;YAN Jin;LYU Jian-Fu(Tianjin Agricultural University,Tianjin 300384,China;State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,266071,China;Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Laboratory for Marine Fisheries Science and Food Production Processes,Laoshan Laboratory,Qingdao 266237,China;Harbin Engineering University,Harbin 150000,China)

机构地区：[1]天津农学院,天津300384 [2]海水养殖生物育种与可持续产出全国重点实验室、中国水产科学研究院黄海水产研究所,山东青岛266071 [3]农业农村部海洋渔业与可持续发展重点实验室、中国水产科学研究院黄海水产研究所,山东青岛266071 [4]崂山实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266237 [5]哈尔滨工程大学,黑龙江哈尔滨150000

出　　处：《海洋与湖沼》2024年第4期960-966,共7页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学青年基金资助项目,32202962号;中国水产科学研究院黄海水产研究所基本科研业务费资助,20603022022015号;国家自然科学基金面上项目资助,52371271号;财政部和农业农村部:国家现代农业产业技术体系专项,CARS-49号。

摘　　要：为深入了解舟形藻(Navicula sp.)生物膜诱导虾夷扇贝(Mizuhopecten yessoensis)幼虫附着变态的机制,选用舟形藻和虾夷扇贝幼虫作为研究对象,研究了4个浓度梯度舟形藻液(1A、10A、100A和1000A)下虾夷扇贝幼虫附着变态率以及幼虫响应舟形藻生物膜诱导的蛋白质组学差异。结果表明,稀释10倍后的藻液(1366 ind./mm^(2))所形成的生物膜对幼虫诱导率最高为45%,与空白组(13%)差异极显著(P<0.01),过高或过低浓度都导致诱导率降低。采用非标记定量技术(4D label free)蛋白质组学分析比较舟形藻生物膜和对照组幼虫发现,经舟形藻生物膜诱导后共316个蛋白质表达显著上调或下调。如谷胱甘肽S转移酶、谷胱甘肽过氧化物酶、组织蛋白酶K、组织蛋白酶L、NAD(P)H氧化酶(形成H2O2)、软骨基质蛋白、胶原蛋白和铁蛋白等。以上这些差异蛋白表达的上下调均与贝类幼虫在附着变态过程中形态学变化息息相关。因此,舟形藻生物膜诱导虾夷扇贝幼虫附着变态的有效成分可能是其产生的胞外多糖,幼虫体内的凝集素检测到舟形藻生物膜表面的多糖后,特异性与多糖结合,最终诱导幼虫完成附着和变态。研究结果可为虾夷扇贝人工培育提供理论依据。To understand the mechanism of the effect of Navicula sp.film substract on the settlement and metamorphosis of Mizuhopecten yessoensis larvae,the settlement and metamorphosis rates of the larvae and the proteomic differences were investigated under four concentrations of Navicula sp.solution(1A,10A,100A,and 1000A;A is the multiple of dilution of the original concentration)and sterilized natural seawater as the control.Results show that the highest metamorphosis rate(45%)was found in Group 10A(1366/mm^(2)),which differed significantly(P<0.01)from that of the control(13%).Too high or too low concentration resulted in a lower induction rate.Using non-labeled quantitative techniques(4D label free)proteomic analysis to compare between Navicula sp.filmed substract and the control group larvae,we found that 316 proteins were significantly up-(198)or down-regulated(118)after induction by the Navicula sp.biofilm.The expressions of cathepsin K,cathepsin L,cartilage matrix proteins,collagen,ferritin,glutathione S-transferase,glutathione peroxidase,and lectin were elevated,while those of heat shock proteins 70 and NAD(P)H oxidase(forming H2O2)proteins were decreased.Proteins such as histone K,histone L,and heat shock proteins were associated with apoptosis,indicating that there is substantial apoptosis during larval settlement and metamorphosis.Proteins such as cartilage matrix proteins,collagen,and ferritin were associated with calcium carbonate shell formation,indicating that larvae induced by Navicula sp.film were in the process of completing the construction of adult shells.Proteins of glutathione S-transferase,glutathione peroxidase,and NAD(P)H oxidase(forming H2O2)were associated with immune function,indicating the maintenance of cellular homeostasis during larval settlement and metamorphosis.C-type lectins and galectin were bound to polysaccharides to trigger larval settlement and metamorphosis.The up-and down-regulation of these differential proteins were closely related to morphological changes in larvae during sett

关 键 词：虾夷扇贝(Mizuhopecten yessoensis) 舟形藻生物膜 变态 蛋白质组学 

分 类 号：S917.4[农业科学—水产科学]