熊本牡蛎幼体、葡萄牙牡蛎幼体和长牡蛎幼体共生菌群构成随宿主发育的演替差异分析  

THE SUCCESSIONAL DIFFERENCE IN STRUCTURE AND COMPOSITION OF COMMENSAL BACTERIAL COMMUNITIES IN THE LARVAE OF CRASSOSTREA SIKAMEA,C.ANGULATA,AND C.GIGAS WITH HOST DEVELOPMENT

作　　者：戴文芳 叶静 刘圣 林志华[1,2] 薛清刚 DAI Wen-Fang;YE Jing;LIU Sheng;LIN Zhi-Hua;XUE Qing-Gang(Ninghai Marine Biological Seed Industry Research Institute,Zhejiang Wanli University,Ningbo 315604,China;Key Laboratory of Aquatic Germplasm Resource of Zhejiang,College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China)

机构地区：[1]浙江万里学院宁海海洋生物种业研究院,浙江宁波315604 [2]浙江万里学院生物与环境学院、浙江省水产种质资源高效利用技术研究重点实验室,浙江宁波315100

出　　处：《海洋与湖沼》2024年第4期967-978,共12页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金项目,32202989号;宁波市“泛3315计划”现代农业创新团队项目,2019~2023。

摘　　要：为了解同一育苗厂育苗的熊本牡蛎幼体、葡萄牙牡蛎幼体和长牡蛎幼体共生菌群随宿主发育的演替是否存在差异,采用16S rRNA高通量测序技术对这3种牡蛎幼体4个发育时期的共生细菌群落构成进行了比较。结果显示,在它们的4个发育时期共鉴定到3484个细菌OTUs,其中在D形幼虫、壳顶幼虫、眼点幼虫和稚贝期共有的OTU数分别占总OTU数的43.1%、24.9%、31.3%和36.8%。4个发育时期的优势菌主要隶属于变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria),而其丰度在不同发育时期存在变化,特别是在细菌属水平差异显著。在4个发育时期3种牡蛎幼体共生菌群多样性有所差异,其中在眼点幼虫期的长牡蛎幼体中菌群多样性最高,而在壳顶幼虫期的熊本牡蛎幼体中菌群多样性最低。值得注意的是,3种牡蛎幼体的共生菌群结构随宿主发育呈现相似的演替变化,但在相同发育时期菌群结构差异显著。进一步对它们各发育时期的共生菌群潜在功能进行比较,发现由共生细菌参与的糖类的生物合成和代谢、脂质代谢、免疫系统等功能通路的丰度在D形幼虫期最高,而参与氨基酸代谢、细胞生长和死亡、其他次级代谢物的生物合成等功能通路的丰度在稚贝期最高但在壳顶幼虫期最低。这些结果表明熊本牡蛎幼体、葡萄牙牡蛎幼体和长牡蛎幼体4个发育时期的共生细菌群落构成存在较大差异,可能受宿主遗传的影响明显。To understand whether there exist differences in the succession of commensal bacterial communities in the larvae of Crassostrea sikamea,C.angulata,and C.gigas with host development,the structure and composition of commensal bacterial communities among the three oyster larvae at four developmental stages were compared via 16S rRNA high-throughput sequencing technology.Results showed that a total of 3484 bacterial OTUs were identified in the four developmental stages of the three oyster larvae,among which the numbers of shared OTUs in the D-shaped,veliger,pediveliger,and spat stages accounted for 43.1%,24.9%,31.3%,and 36.8%of total OTUs,respectively.The dominant bacteria at four developmental stages were attached to Proteobacteria,Bacteroidetes,and Actinobacteria,and their abundance varied in different larval developmental stages.In particular,significant differences were observed at the bacterial genus level.The diversity of commensal bacterial communities of the three oyster larvae was different in four developmental stages.The bacterial diversity was the highest in C.gigas larvae at pediveliger stage,but was the lowest in C.sikamea larvae at veliger stage.Notably,the structure of commensal bacterial communities among the three oyster larvae showed similar succession change with host development,and the significant variation was found at same development stage.Furthermore,comparison of the potential functions of its commensal bacterial communities at different developmental stages showed that the abundances of functional pathways involved in the carbohydrate biosynthesis and metabolism,lipid metabolism,and immune system were the highest at the D-shaped stage,whereas those involved in the amino acid metabolism,cell growth and death,and biosynthesis of other secondary metabolites were the highest at the spat stage but the lowest at veliger stage.These results indicate that there were significant differences in the structure and composition of commensal bacterial communities in the larvae of C.sikamea,C.angulata,and

关 键 词：牡蛎幼体 共生菌群 发育时期 构成特征 演替变化 

分 类 号：Q938.8[生物学—微生物学] S917.1[农业科学—水产科学] S965