基于ND5序列的西北太平洋海域四种弹涂鱼遗传变异比较研究  

GENETIC DIVERSITY OF FOUR MUDSKIPPER(GOBIIDAE)IN THE NORTHWEST PACIFIC BASED ON mtDNA ND5 GENES

作　　者：肖东东 吴增满 赵林林 宋娜[1] XIAO Dong-Dong;WU Zeng-Man;ZHAO Lin-Lin;SONG Na(The Key Laborary of Mariculture(Ocean University of China),Ministry of Education,Qingdao 266003,China;The First Institute of Oceanography,Ministry of Natural Resources,Qingdao 266061,China)

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]自然资源部第一海洋研究所,山东青岛266061

出　　处：《海洋与湖沼》2024年第4期1037-1047,共11页Oceanologia Et Limnologia Sinica

基　　金：国家重点研究发展计划项目,2019YFD0901301号。

摘　　要：弹涂鱼是一类两栖性鱼类的统称,具有重要的经济价值和生态价值。本研究基于线粒体ND5基因序列比较分析了西北太平洋地区4种弹涂鱼,即弹涂鱼(Periophthalmus modestus)、大鳍弹涂鱼(Periophthalmus magnuspinnatus)、大弹涂鱼(Boleophthalmus pectinirostris)和薄氏大弹涂鱼(Boleophthalmus boddarti)的群体遗传多样性和遗传结构。研究结果显示,在长度549 bp的线粒体ND5序列中,单倍型多样度:弹涂鱼>薄氏大弹涂鱼>大弹涂鱼>大鳍弹涂鱼,核苷酸多样度:大弹涂鱼>弹涂鱼>薄氏大弹涂鱼>大鳍弹涂鱼。此外,基于单倍型序列构建的邻接关系树可以看出,4种弹涂鱼的单倍型可各自聚成单系,但种内均不存在显著的谱系结构。群体间的遗传分化指数(Fst)表明弹涂鱼的中日群体间存在显著分化,大弹涂鱼湛江、厦门、温州群体两两之间遗传分化与其地理距离呈正比。分子方差分析(AMOVA)的结果显示分子变异主要来自于群体内,表明其群体内多样性较高。中性检验、核苷酸不配对分布以及贝叶斯天际线分析结果均表明弹涂鱼和大弹涂鱼在更新世末期可能经历了群体扩张事件。该研究对西北太平洋海域的4种弹涂鱼的遗传变异进行了全面的比较分析,研究结果可为弹涂鱼鱼类的资源保护与合理利用提供基础资料。Mudskippers are a collective term for amphibious fish widely distributed in the Northwest Pacific,possessing significant economic and ecological value.A comparative analysis was conducted on the population genetic diversity and genetic structure of four mudskipper species in the Northwest Pacific,namely,Periophthalmus modestus,Periophthalmus magnuspinnatus,Boleophthalmus pectinirostris,and Boleophthalmus boddarti,based on the mitochondrial ND5 gene sequence.Results show that,in the 549 bp mitochondrial ND5 sequence,the haplotype diversity of P.modestus was the highest,followed by B.boddarti,B.pectinirostris,and P.magnuspinnatus.The nucleotide diversity of B.pectinirostris was the highest,followed by P.modestus,B.boddarti,and P.magnuspinnatus.The constructed neighbor-joining tree based on haplotypes showed that the above four mudskipper species were clustered into separate clades,but there was no significant phylogenetic structure within each species.The Fst(fixation index,a measure of population differentiation in genetic structure)results of P.modestus revealed significant differentiation between the Chinese and Japanese populations.For B.pectinirostris,the Fst values among populations Zhanjiang,Xiamen,and Wenzhou were proportional to geographical distance.In addition,as AMOVA(analysis of molecular variance)indicate,the molecular variation came mainly from within populations,indicating a relatively high level of differentiation within populations.Meanwhile,the neutral tests,nucleotide mismatch distribution,and Bayesian skyline plot analysis all suggested that mudskipper and B.pectinirostris might have experienced a population expansion event at the end of the Pleistocene.This study provided a comprehensively comparative analysis of genetic variation of four mudskipper species in the Northwest Pacific,and offered fundamental data for their resource conservation and rational utilization.

关 键 词：西北太平洋 4种弹涂鱼类 遗传多样性 遗传结构 

分 类 号：Q789[生物学—分子生物学] S931[农业科学—渔业资源] Q953[农业科学—水产科学]