超高效液相色谱法同时测定安宫降压丸中9种成分含量  

Simultaneous Determination of Nine Components in Angong Jiangya Pills by UPLC

作　　者：邵晓玮 王洪明 曹洪杰 安静 李玉平 SHAO Xiaowei;WANG Hongming;CAO Hongjie;AN Jing;LI Yuping(Binzhou Municipal Testing Center,Binzhou,Shandong,China 256600;Binzhou Municipal Key Laboratory for Chemical Drug Research and Development and Quality Control,Binzhou,Shandong,China 256600;Binzhou Medical University Hospital,Binzhou,Shandong,China 256600)

机构地区：[1]山东省滨州市检验检测中心,山东滨州256600 [2]山东省滨州市化学药物研发与质量控制重点实验室<筹>,山东滨州256600 [3]山东省滨州市医学院附属医院,山东滨州256600

出　　处：《中国药业》2024年第15期66-69,共4页China Pharmaceuticals

摘　　要：目的 建立同时测定安宫降压丸中栀子苷、盐酸黄连碱、表小檗碱、黄芩苷、盐酸小檗碱、盐酸巴马汀、汉黄芩苷、黄芩素、汉黄芩素9种指标性成分含量的超高效液相色谱法。方法 色谱柱为Waters Acquity UPLC C_(18)柱(100 mm×2.1 mm,1.7μm),流动相为乙腈-0.2%磷酸溶液(梯度洗脱),流速为0.5 mL/min,检测波长为254 nm,柱温为35℃,进样量为1μL。结果 9种成分在各自质量浓度范围内与峰面积线性关系良好(r≥0.999 5);精密度、稳定性、重复性试验结果的RSD均小于2.0%;平均加样回收率分别为99.82%,98.91%,99.09%,99.09%,98.77%,99.25%,99.16%,98.17%,99.40%,RSD分别为0.56%,0.56%,0.81%,0.97%,0.79%,0.81%,0.84%,1.19%,0.83%(n=6)。样品中9种成分的含量分别为1.82,0.56,0.68,4.52,2.36,0.54,0.85,0.37,0.32 mg/g(n=9)。结论 该方法快速高效、准确度高、专属性强,可用于安宫降压丸中9种指标性成分的含量测定。Objective To establish an ultra-high performance liquid chromatography (UPLC) method for simultaneous determination of nine index components (geniposide,coptisine hydrochloride,epiberberine,baicalin,berberine chloride,berberine chloride,wogonoside,and baicalein) in Angong Jiangya Pills.MethodsThe chromatography column was Waters Equity UPLC C_(18)column (100 mm×2.1 mm,1.7μm),the mobile phase was acetonitrile -0.2% phosphoric acid solution (gradient elution),the flow rate was 0.5 mL/min,the detection wavelength was 254 nm,the column temperature was 35℃,and the injection volume was 1μL.ResultsThe nine components showed good linear relationships with peak area within their respective mass concentration ranges (r≥0.999 5).The RSDs of precision,stability,and repeatability test results were all lower than 2.0%.The average recoveries of nine components were 99.82%,98.91%,99.09%,99.09%,98.77%,99.25%,99.16%,98.17%,and 99.40%,with RSDs of 0.56%,0.56%,0.81%,0.97%,0.79%,0.81%,0.84%,1.19%,and 0.83%(n=6),respectively.The contents of nine components in the sample were 1.82,0.56,0.68,4.52,2.36,0.54,0.85,0.37,and 0.32 mg/g (n=9),respectively.ConclusionThe method is quick,sensitive,and accurate,and it has strong specificity,which can be used for the content determination of nine components in Angong Jiangya Pills.

关 键 词：安宫降压丸 超高效液相色谱法 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学] R286.0