化皮刺参组织中盾纤毛虫的分离与鉴定  

Isolation and identification of scuticociliate in the tissues of ulceration epidermis of Apostichopus japonicus.

作　　者：邓鸿圣 张荣伟 陈毅 赵小然 DENG Hongsheng;ZHANG Rongwei;CHEN Yi;ZHAO Xiaoran(Aquatic Animal Hospital,Dalian Ocean University,Dalian 116023,China;Laboratory of Aquatic Animal Nutrition and Feed,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]大连海洋大学水生动物医院,辽宁大连116023 [2]大连海洋大学水产动物营养与饲料实验室,辽宁大连116023

出　　处：《养殖与饲料》2024年第8期57-61,共5页Animals Breeding and Feed

基　　金：辽宁省教育厅高等学校基本科研项目(LJKMZ20221121)。

摘　　要：[目的]对化皮刺参(Apostichopus japonicus)病例中的寄生虫进行分离鉴定,分析其致病因素,为刺参盾纤毛虫病诊断和防控提供参考依据。[方法]对辽宁某养殖场送检的化皮刺参表皮、体壁、肌肉、体腔液、呼吸树、肠道进行镜检,将观察到的纤毛虫离体培养,并进行18S rDNA测序。[结果]病参各组织器官观察到纤毛虫H-1、H-2、H-3和H-4,H-4通过形态学判断为后口虫,成功离体培养出H-3与镜检未观察到的H-5。纤毛虫H-3与H-5的18S rDNA序列比对结果显示为海洋尾丝虫(Uronema marinum)。[结论]本病例中刺参各组织寄生多种纤毛虫,体腔液中寄生海洋尾丝虫,呼吸树中寄生后口虫和海洋尾丝虫。[Objectives]The parasites in the cases of Apostichopus japonicus were isolated and identified,and its pathogenic factors were analyzed to provide a reference for the diagnosis,prevention and control of scuticociliate in A.japonicus.[Methods]Microscopic examination was conducted on the epidermis,body wall,muscles,body cavity fluid,respiratory tree,and intestines of the A.japonicus sent from a farm in Liaoning Province for inspection.The observed ciliates were cultured in vitro and its 18S rDNA were sequenced.[Results]The ciliate H-1,H-2,H-3 and H-4 was observed in various tissues and organs of the diseased ginseng.The ciliate H-4 was identified as a Boveria Stevens based on morphology.The ciliate H-3 and H-5 not observed under microscopy were successfully cultured in vitro.The alignment of the 18S rDNA sequences of ciliates H-3 and H-5 showed that they were U.marinum.[Conclusions]In this case,various scuticociliate were parasitized in the tissues of A.japonicus,while U.marinum were parasitized in the body cavity fluid,and Boveria Stevens and U.marinum were parasitized in the respiratory trees.

关 键 词：化皮刺参 盾纤毛虫 18S rDNA 离体培养 后口虫 海洋尾丝虫 

分 类 号：S947.9[农业科学—水产养殖]