静电自组装法构建抗菌抗凝涂层的研究  

Electrostatic Self-assembly Method for the Preparation of Antibacterial and Anticoagulant Surface Coating

作　　者：李鹏程 魏嘉佳 孟昊天 王文轩 李佳峻 李达[1] 涂秋芬[1] LI Pengcheng;WEI Jiajia;MENG Haotian;WANG Wenxuan;LI Jiajun;LI Da;TU Qiufen(School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China)

机构地区：[1]西南交通大学材料科学与工程学院,成都610031

出　　处：《材料导报》2024年第14期255-263,共9页Materials Reports

摘　　要：在临床治疗过程中,血栓形成以及细菌污染导致的高病发率和高致死率严重制约了血液接触类医疗器械的应用和发展。本研究基于溶菌酶(LZM)优异的抗菌性能以及在较广pH范围内所带的正电荷,通过静电自组装,将血液相容性良好的带负电荷的聚苯乙烯磺酸钠(PSS)吸附到材料表面,构建具有抗菌、抗凝性能的生物功能性涂层。傅里叶红外光谱(FTIR)、X射线光电子能谱(XPS)、表面电位、水接触角(WCA)等材料学评价结果证明PDA@LZM/PSS涂层被成功构建。细菌实验的结果表明,PDA@LZM/PSS涂层具有优异的革兰氏阴性菌和革兰氏阳性菌灭杀效果。血液实验结果表明,涂层表面具有优异的抗血小板粘附、激活以及抗血栓形成的性能。细胞实验结果表明,涂层没有细胞毒性,可以应用于血液接触类器械表面改性。涂层稳定性实验结果表明,涂层能够在7 d内维持较为稳定的抗菌、抗凝特性。该涂层的制备方法简单快速,可应用于血液接触类器械表面抗菌、抗凝血改性,可有效降低血栓形成及细菌感染的风险率。The high morbidity and mortality caused by thrombosis and pathogen contamination have severely restricted the application and development of blood-contact medical devices in clinical treatment.In this work,based on the outstanding antibacterial properties of lysozyme(LZM)and its positive charge over a wide pH range,negatively charged polystyrene sodium sulfonate(PSS)with excellent blood compatibility was electrostatically self-assembled to the surface of the material,creating an antibacterial and anticoagulant bio-functional coating.The results of material characterisation,including Fourier transform infrared spectroscopy(FTIR),X-ray photoelectron spectroscopy(XPS),surface potential,and water contact angle(WCA),demonstrated the successful fabrication of PDA@LZM/PSS coating.Bacterial experiments revealed that the PDA@LZM/PSS coating had an outstanding killing effect on both Gram-negative and Gram-positive bacteria.Blood experiments showed that the coated surface had excellent anti-platelet adhesion,activation,and anti-thrombosis properties.The results of cell experiments indicated that the coating was non-cytotoxic and could be applied to the surface modification of blood-contact devices.Lastly,stability testing indicated that the coating could maintain its antibacterial and anticoagulant capabilities relatively stable for seven days.The coating preparation was simple and fast,allowing it to be applied to antibacterial and anticoagulant modification on the surface of blood-contact instruments,thereby reducing the risk of thrombus formation and bacterial infection.

关 键 词：静电自组装 溶菌酶(LZM) 聚苯乙烯磺酸钠(PSS) 抗菌 抗凝 表面改性 

分 类 号：O647[理学—物理化学]