PEGMA刷微图案诱导聚苯乙烯纳米颗粒阵列化  

The Fabrication of Polystyrene Nanoparticle Microarrays Through the Micropatterned PEGMA Brush Induced Assembly

作　　者：高浩 魏中华 邓佳 陈涛 赵海利 GAO Hao;WEI Zhonghua;DENG Jia;CHEN Tao;ZHAO Haili(Faculty of Chemical Engineering,Kunming University of Science and Technology,Kunming 650504,China)

机构地区：[1]昆明理工大学化学工程学院,昆明650504

出　　处：《材料导报》2024年第14期270-276,共7页Materials Reports

基　　金：国家自然科学基金(52103262)。

摘　　要：基于光引发原子转移自由基聚合(ATRP)反应,在疏水性引发剂表面制备亲水性聚乙二醇甲基丙烯酸酯(PEGMA)聚合物刷微图案,以PEGMA聚合物刷图案化表面为模板,借助亲疏水区域间表面能的差异使聚苯乙烯(PS)纳米颗粒在亲水区域精准组装制备纳米颗粒微阵列。采用X射线光电子能谱仪(XPS)与拉曼光谱仪(Raman)对各阶段基体表面化学组分进行表征,结果表明,聚多巴胺/3-(三甲氧基硅烷基)丙基2-溴-2-甲基丙酸酯(PDA/SiBr)复合涂层、PEGMA聚合物刷以及PS纳米颗粒微阵列被成功制备。采用扫描电子显微镜(SEM)和光学显微镜对PEGMA聚合物刷图案化表面及PS纳米颗粒阵列化表面进行观察,结果表明PS纳米颗粒在PEGMA聚合物刷微图案区域选择性组装。研究了PS纳米颗粒微阵列表面BSA蛋白吸附行为,荧光图像结果表明PS纳米颗粒在PEGMA聚合物刷微图案表面组装后可以有效促进BSA蛋白在该区域的黏附。相关研究工作拓展了图案化聚合物刷在纳米颗粒微阵列制备中的应用,为纳米颗粒在高质量生物微阵列、生物探针等领域的应用提供了技术支持。Hydrophilic poly(ethylene glycol)methacrylate(PEGMA)brush micropatterns were prepared on the hydrophobic initiator surface based on the photoinitiated atom transfer radical polymerization(ATRP)reaction.With PEGMA brushes micropatterned substrate acted as a template,the precise assembly of polystyrene(PS)nanoparticles in the hydrophilic region was achieved by utilizing the surface energy difference between hydrophilic and hydrophobic regions,enabling the fabrication of nanoparticle microarrays.X-ray photoelectron spectroscopy(XPS)tests revealed that the covalent immobilization of 3-(Trimethoxysilyl)propyl 2-bromo-2-methylpropanoate(SiBr)initiator on the polydopamine(PDA)surface was achieved.Raman spectroscopy tests indicated the growth of PEGMA brushes on the initiator surface and the assembly of PS nanoparticles on the PEGMA brushes surface.Optical microscopy and scanning electron microscopy(SEM)characterizations showed the selective assembly of PS nanoparticles on the PEGMA brush regions,leading to the successful fabrication of nanoparticle microarrays.Fluorescein thiocyanate-labeled bovine serum protein(FITC-BSA)was applied to detect the protein adsorption behavior of PS nanoparticles,and the results demonstrated the PS nanoparticles could effectively promote the adhesion of BSA protein on the antifouling PEGMA brush region.The presented fabrication technique shows great potential in the fabrication of nanoparticle microarrays with complex structures and provides technical support for the application of nanoparticles in high-throughput bio-microarrays and bioprobes.

关 键 词：聚乙二醇甲基丙烯酸酯(PEGMA)刷微图案 亲疏水反差特性 聚苯乙烯(PS)纳米颗粒 微阵列 蛋白吸附 

分 类 号：TB303[一般工业技术—材料科学与工程]