基于半胱氨酸氧化修饰蛋白质组学研究高压处理对冷藏滩羊肉应力松弛特性的影响  

Effect of High-Pressure Treatment on Stress Relaxation Characteristics of Refrigerated Tan Sheep Muscle Investigated by Cysteine Oxidative Modification Proteomics

作　　者：高钰茸 卜宁霞 张瑞[1] 王素也 刘敦华[1] GAO Yurong;BU Ningxia;ZHANG Rui;WANG Suye;LIU Dunhua(College of Food Science and Engineering,Ningxia University,Yinchuan 750021,China;College of Animal Science and Technology,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学食品科学与工程学院,宁夏银川750021 [2]宁夏大学动物科技学院,宁夏银川750021

出　　处：《肉类研究》2024年第6期9-18,共10页Meat Research

基　　金：宁夏自然科学基金项目(2021AAC03013,2022AAC02021);宁夏回族自治区农业高质量发展与生态保护科技创新示范项目(NGSB-2021-6-05)。

摘　　要：通过半胱氨酸(Cys)氧化修饰蛋白质组学探究不同高压处理(200 MPa/500 MPa、18℃、15 min)滩羊(4℃冷藏1、3、7 d)肌肉蛋白半胱氨酸的氧化修饰与应力松弛特性之间的相关性。结果表明:200 MPa和500 MPa高压处理的肌肉硬度和弹性显著高于对照(NT)组(P<0.05);高压处理组应力松弛特性指标普遍高于NT组,贮藏期间,500 MPa高压处理组羰基含量显著高于200 MPa高压处理组,而总巯基含量显著低于200 MPa高压处理组(P<0.05);半胱氨酸氧化修饰蛋白质组学分析结果显示,3组样本中共鉴定出388个显著差异氧化位点(significantly differential oxidation sites,SDOS),其中,15个SDOS与至少1个应力松弛特性指标呈显著相关性(P<0.05、P<0.01),这些位点涉及肌联蛋白(Cys32705、Cys29171、Cys26405、Cys32358)、伴肌动蛋白(Cys5628)、肌球蛋白结合蛋白H(Cys481)、肌球蛋白重链8(Cys403)、膜联蛋白(Cys292)、肌球蛋白重链11(Cys701)、L-乳酸脱氢酶(Cys315)、糖原磷酸化酶(Cys496)、磷酸葡萄糖变位酶1(Cys407、Cys134)、丙酮酸激酶(Cys510)及钙转运ATP酶(Cys651)。综上所述,高压处理可诱导结构蛋白的部分氧化位点发生氧化修饰,使蛋白变性和聚集,进而降低蛋白本身的降解程度,诱导滩羊肉贮藏期间的硬度增加。The current study used cysteine oxidative modification proteomics to investigate the correlation between oxidative modification of cysteine(Cys) and stress relaxation characteristics in Tan sheep muscle subjected to high-pressure(HP)treatments at 200 or 500 MPa for 15 min at 18 ℃ and stored for 1,3 and 7 days at 4 ℃.The results showed that muscle hardness and elasticity were significantly higher following HP treatments compared to the non-treated(NT) group(P < 0.05).Most stress relaxation indexes were higher in the HP-treated groups than in the CN group.During all storage periods,the carbonyl value of the 500 MPa treated group was significantly higher,and the total sulfhydryl content was significantly lower than that of the 200 MPa treated group(P < 0.05).A total of 388 significantly differential oxidation sites were identified,of which 15 were significantly correlated with at least one indicator of stress relaxation(P < 0.05),involving myosin(Cys32705,Cys29171,Cys26405,and Cys32358),concomitant actin(Cys5628),myosin binding protein H(Cys481),myosin heavy chain 8(Cys403),membrane-associated protein(Cys292),myosin heavy chain 11(Cys701),L-lactate dehydrogenase(Cys315),glycogen phosphorylase(Cys496),phosphoglucose translocase 1(Cys407 and Cys134),pyruvate kinase(Cys510)and calcium transporter ATPase(Cy651).Therefore,high-pressure treatment induces oxidative modification of some oxidation sites of structural proteins in sheep muscle and consequent protein denaturation and aggregation,in turn reducing the degradation of proteins and inducing a decrease the hardness of Tan sheep meat.

关 键 词：高压处理 滩羊肉 应力松弛特性 蛋白质氧化 半胱氨酸氧化修饰蛋白质组学 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]