短期存贮温度对生物絮团反硝化性能的影响  

Effect of short-term storage temperature ondenitrification performance of bioflocs

作　　者：肖定东 刘文畅 郭衍硕[1] 罗国芝 谭洪新 XIAO Dingdong;LIU Wenchang;GUO Yanshuo;LUO Guozhi;TAN Hongxin(Shanghai Collaborative Innovation Center for Cultivating Elite Breed Aquatic andGreen-culture of Aquaculture Animals,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Germplasm Resourcces,Ministry ofAgriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海市水产动物良种创制与绿色协同创新中心,上海201306 [2]上海海洋大学上海水产养殖工程技术研究中心,上海201306 [3]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《渔业现代化》2024年第4期37-47,共11页Fishery Modernization

基　　金：上海市青年人才计划启明星培育(扬帆专项)“工业化水产养殖系统中絮团颗粒对南美白对虾的生物效应研究(22YF1416400)”。

摘　　要：为探究存贮温度对生物絮团反硝化性能的影响,试验收集凡纳滨对虾( Litopenaeus vannamei )养殖池中的生物絮团,分别密封保存于室温25 ℃(T25组)、低温4 ℃(T4组)、 冷冻-20 ℃(T-20组)和超低温冷冻-80 ℃(T-80组)条件下,为期28 d。初始收集到的生物絮团对硝酸盐氮的去除速率为0.71±0.03 mg /(L·h)。保存前7 d,仅T-80组的生物絮团去除速率出现衰减。保存时长超过14 d以后,仅T4组的生物絮团反硝化性能未衰减。保存至第28天,T4组生物絮团对硝酸盐氮的去除速率为0.72±0.01 mg/(L·h),显著高于T25组0.52±0.02 mg/(L·h)、T-20组0.57±0.03 mg/(L·h)和T-80组0.52±0.02 mg/(L·h)。显微结构观察表明,生物絮团保存 7 d与初始相比,T4组和T25组未出现明显变化,死亡细胞比例增加缓慢;保存至第28天,T4组生物絮团轻微变质,死亡细胞比例低,其他组别均出现生物絮团严重变质、死亡细胞比例较高的情况。综上,4 ℃是生物絮团反硝化性能短期保存的适宜温度。To explore the effect of storage temperature on the denitrification performance of bioflocs,the bioflocs in Litopenaeus vannamei culture ponds were collected and sealed at room temperature 25 ℃ (T25 group),low temperature 4 ℃ (T4 group),freezing-20 ℃ (T-20 group) and ultra-low temperature freezing-80 ℃(T-80 group) for 28 days.The removal rate of nitrate nitrogen by the initial collected bioflocs was 0.71±0.03 mg/(L·h).During the initial 7 days of storage,only the T-80 group exhibited a decrease in the bioflocs' denitrification rate.After 14 days of storage,the denitrification performance of the bioflocs in the T4 group remained stable.On the 28th day,the removal rate of nitrate nitrogen by T4 group was 0.72±0.01 mg/(L·h),which was significantly higher than that of T25 group (0.52±0.02 mg/(L·h)),T-20 group (0.57± 0.03 mg/(L·h) ) and T-80 group (0.52±0.02 mg/(L·h)).Microstructure observation showed that there was no significant change in T4 group and T25 group on the 7th day of biofloc preservation compared with the initial,and the proportion of dead cells increased slowly.On the 28 th day,the bioflocs in the T4 group were slightly deteriorated,and the proportion of dead cells was low.The bioflocs in other groups were severely deteriorated and the proportion of dead cells was high.In summary,4 ℃ is the suitable temperature for short-term preservation of denitrification performance of bioflocs.

关 键 词：生物絮团 反硝化性能 保存温度 显微结构 

分 类 号：S966.12[农业科学—水产养殖]