番茄BURP结构域基因家族全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of Tomato BURP Structural Domain Gene Family

作　　者：张福林 奚瑞 刘宇翔 陈兆龙 余庆辉[1] 李宁[1] ZHANG Fulin;XI Rui;LIU Yuxiang;CHEN Zhaolong;YU Qinghui;LI Ning(Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables,Institute of Horticultural Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;College of Horticulture,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业科学院园艺作物研究所,新疆特色果蔬基因组研究与遗传改良重点实验室,乌鲁木齐830091 [2]新疆农业大学园艺学院,乌鲁木齐830052

出　　处：《中国农业科技导报》2024年第8期51-62,共12页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(32260763);新疆农业科学院科技创新专项孵化项目(xjkcpy-2021001)。

摘　　要：BURP结构域蛋白为植物所特有,在植物生长发育和胁迫响应中起着重要作用。为鉴定番茄BURP结构域基因(SlBURP)及分析其表达,从番茄基因组中鉴定出12个SlBURP基因,系统进化分析将其分为7个亚族,不同亚族的保守基序和基因结构有明显差异。SlBURPs启动子序列中广泛存在胁迫、生长、激素、光响应元件,表明该家族基因在对非生物胁迫的响应中有重要作用。共线性分析表明,番茄SlBURP基因和拟南芥BURP基因家族成员来自同一祖先。RT-qPCR表明,SlBURP6、SlBURP7、SlBURP8、SlBURP12在叶中大量表达;SlBURP2、SlBURP6、SlBURP7、SlBURP8在盐处理后转录水平上升;在植物调节剂处理下SlBURP5、SlBURP8、SlBURP9、SlBURP12基因在果实发育期的转录水平均有上升。综上所述,SlBURP基因在番茄生长发育和逆境胁迫中起着重要作用。BURP domain proteins are unique to plants and play different roles in plant stress,growth and development.To identify BURP domain genes(SlBURP)and their expression in tomato,a total of 12 SlBURP genes were identified in tomato.The phylogenetic analysis classified 12 SlBURP genes into 7 subfamilies,which had significant differences in conserved motifs and gene structures among the different subfamilies.The extensive presence of stress,growth,hormone,and light-responsive elements in the promoter sequences of SlBURPs suggested that this family had crucial role in abiotic stress.Covariance analysis indicated that members of tomato and Arabidopsis BURP gene families were derived from the same ancestor.The RT-qPCR results showed that the expression levels of SlBURP6,SlBURP7,SlBURP8 and SlBURP12 were abundantly in leaves;the expression levels of SlBURP2,SlBURP6,SlBURP7 and SlBURP8 increased after salt treatment;the expression levels of SlBURP5,SlBURP8,SlBURP9 and SlBURP12 increased during fruit development under plant regulator treatment.In summary,SlBURP genes played important roles in both stress and growth of tomato.

关 键 词：番茄 BURP基因家族 果实发育 盐胁迫 表达分析 

分 类 号：S641.2[农业科学—蔬菜学]