外周血单个核细胞SORT1基因检测在小儿脓毒血症诊断中的临床意义  

Clinical significance of the detection of SORT1 gene in peripheral blood mononuclear cells for the diagnosis of pediatric sepsis

作　　者：李彦 王进朝 张根豪[3] LI Yana;WANG Jinchao;ZHANG Genhao(Department of Blood Transfusion,Anyang People′s Hospital,Anyang 455000,Henan;Department of Clinical Laboratory,Anyang People′s Hospital,Anyang 455000,Henan;Department of Blood Transfusion,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,Henan,China)

机构地区：[1]安阳市人民医院输血科,河南安阳455000 [2]安阳市人民医院检验科,河南安阳455000 [3]郑州大学第一附属医院输血科,郑州450052

出　　处：《临床检验杂志》2024年第7期492-496,共5页Chinese Journal of Clinical Laboratory Science

摘　　要：目的探讨分拣蛋白1(sortilin 1,SORT1)基因作为小儿脓毒血症新的诊断生物学标志物的临床应用价值。方法从GEO数据库中获取了3个小儿脓毒血症相关的微阵列数据集(GSE13904、GSE26378和GSE26440),分析SORT1基因在正常样本和脓毒血症样本中的表达差异,并利用实时荧光定量PCR(qRT-PCR)在65例临床外周血样本中对其进行验证。使用基因集富集分析(GSEA)探讨SORT1基因相关的分子机制和生物学过程。结果在GSE13904、GSE26378、GSE26440数据集和临床外周血样本中,SORT1基因在脓毒血症样本中的表达水平均高于正常样本。与常用的炎症指标如降钙素原(PCT)、C反应蛋白(CRP)、白细胞(WBC)和中性粒细胞百分比(Neu%)相比,SORT1基因对小儿脓毒血症具有更高的诊断效能(AUCROC=0.863)。此外,SORT1基因可通过TNF-α/NF-κB信号通路参与小儿脓毒血症的发生、发展。结论SORT1基因对小儿脓毒血症具有一定的诊断价值,但需要在临床工作中进一步证实。Objective To explore the clinical significance of sortilin 1(SORT1)gene as a new diagnostic biomarker for pediatric sep-sis.Methods Three pediatric sepsis-related microarray datasets,including GSE13904,GSE26378,and GSE26440,were obtained from the gene expression omnibus(GEO)database to analyze the expression difference of SORT1 gene between normal and sepsis sam-ples.Then,65 clinical samples were used to validate the difference through qRT-PCR.The molecular mechanism and biological process associated with SORT1 gene were explored by the gene set enrichment analysis(GSEA).Results The expression levels of SORT1 gene in the sepsis samples of GSE13904,GSE26378,and GSE26440 datasets and clinical samples were higher than that in normal samples.Compared with commonly used inflammatory markers such as procalcitonin(PCT),C-reactive protein(CRP),white blood cell(WBC),and the percentage of neutrophils(Neu%),SORT1 gene showed better diagnostic ability for pediatric sepsis and the area under the receiver operating characteristic(ROC)curve(AUCROC)was 0.863.SORT1 gene may be involved in the occurrence and de-velopment of pediatric sepsis through the TNF-α/NF-κB signaling pathway.Conclusion SORT1 gene may have certain diagnostic val-ue for pediatric sepsis,which needs to be further confirmed in clinical practice.

关 键 词：小儿脓毒血症 分拣蛋白1基因 ROC曲线 基因集富集分析 

分 类 号：R515.3[医药卫生—内科学]