SVT对EM大鼠炎症反应及异位内膜细胞凋亡的影响  

Mechanism of the effect of SVT on inflammatory response and ectopic endometrial cell apoptosis in EM rats

作　　者：张晶晶 朱乐玫[2] 刘娟[2] 黄丽 ZHANG Jingjing;ZHU Lemei;LIU Juan;HUANG Li(The Maternal and Child Health Hospital of Hunan Province,Changsha 410008,Hunan,China;Changsha Medical School,Changsha 410219,Hunan,China)

机构地区：[1]湖南省妇幼保健院,湖南长沙410008 [2]长沙医学院,湖南长沙410219

出　　处：《中南医学科学杂志》2024年第4期534-538,共5页Medical Science Journal of Central South China

基　　金：湖南省教育厅重点项目(20A056);湖南省卫生健康委科研课题(202112041226)。

摘　　要：目的探究辛伐他汀(SVT)对子宫内膜异位症(EM)大鼠炎症反应及异位内膜细胞凋亡的影响。方法将48只SD雌性大鼠随机均分成对照组、EM组、SVT组及SVT+脂多糖(LPS)组。采用组织学分析检测各组大鼠子宫内膜异位灶体积和粘连评分;ELISA检测大鼠血清白细胞介素-6(IL-6)、血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)水平;HE染色观察大鼠子宫内膜组织病理学变化;TUNEL染色检测内膜细胞凋亡情况;Western blotting法检测大鼠子宫内膜组织TNF-α/核因子-κB(NF-κB)通路相关蛋白表达水平。结果与对照组大鼠比较,EM组大鼠子宫内膜组织结构紊乱、炎症细胞浸润明显,子宫内膜异位灶体积增加,腹腔粘连评分及血清IL-6、VEGF、TNF-α水平升高,子宫内膜细胞凋亡率及TNF-α/NF-κB通路激活水平也升高(P<0.05)。与EM组比较,SVT组大鼠子宫内膜上皮结构显著恢复,子宫内膜异位灶体积减少,腹腔粘连评分及血清IL-6、VEGF和TNF-α水平降低,TNF-α/NF-κB通路激活水平受到抑制而异位子宫内膜细胞凋亡率进一步升高(P<0.05);而SVT+LPS组细胞凋亡率较SVT组下降(P<0.05)。结论辛伐他汀通过抑制TNF-α/NF-κB信号通路减轻EM大鼠的炎症反应,促进异位内膜细胞凋亡。Aim To Explore the effect of simvastatin(SVT)on inflammatory response and apoptosis of ectopic endometrial cells in rats with endometriosis(EM).Methods 48 SD female rats were randomly divided into the control group,the EM group,the SVT group and the SVT+lipopolysaccharide(LPS)group.Histological analysis was used to detect the volume and adhesion score of endometriosis lesions in each group of rats.ELISA kits were used for detecting interleukin-6(IL-6),vascular endothelial growth factor(VEGF),and tumor necrosis factor-α(TNF-α)in rat serum.HE staining was used to observe the pathological changes of rat endometrium tissue.TUNEL staining was used to detect endometrial cell apoptosis.Western blotting method was used to detect TNF-α/nuclear factor-κB(NF-κB)pathway related proteins.Results Compared with the control group,rats in EM group showed distorted intimal structure and obvious inflammatory cell infiltration,along with increased endometriotic lesion volume and abdominal adhesion score,increased serum IL-6,VEGF and TNF-αlevels,enhanced ectopic endometrial cell apoptosis and TNF-α/NF-κB signal pathway activation(P<0.05).Compared with the EM group,the SVT treatment significantly restored the endometrial epithelial structure,and decreased the volume of endometriosis lesions and abdominal adhesion score,as well as serum IL-6,VEGF and TNF-αlevels were decreased.TNF-α/NF-κB signal pathway was inhibited,whereas ectopic endometrial cell apoptosis was further enhanced(P<0.05).The apoptosis index of SVT+LPS group was decreased compared with the SVT group(P<0.05).Conclusion Simvastatin reduces the inflammatory response in EM rats and promotes apoptosis of ectopic endometrial cells by inhibiting TNF-α/NF-κB signaling pathway.

关 键 词：辛伐他汀 子宫内膜异位症 炎症反应 细胞凋亡 肿瘤坏死因子-Α 核因子-ΚB 

分 类 号：R711.71[医药卫生—妇产科学]