蜂胶薄荷糖的安全性毒理学评价  

Safety and Toxicological Evaluation of Propolis Mints

作　　者：张延峰 何浩麟 黄桂芬 方海红 ZHANG Yanfeng;HE Haolin;HUANG Guifen;FANG Haihong(School of Pharmacy,Jiangxi Science and Technology Normal University,Nanchang 330013,China;Hangzhou Meleha Biotech Lt,Hangzhou 310000,China)

机构地区：[1]江西科技师范大学药学院,南昌330013 [2]杭州麦乐哈生物科技有限公司,杭州310000

出　　处：《江西中医药大学学报》2024年第4期89-94,113,共7页Journal of Jiangxi University of Chinese Medicine

摘　　要：目的:评价蜂胶薄荷糖的食用安全性及健康风险。方法:对蜂胶薄荷糖进行急性经口毒性试验、遗传毒性试验及28 d经口毒性试验。结果:急性经口毒性试验显示,给药后未观察到受试小鼠出现中毒或死亡症状,急性经口的半数致死量(LD50)>10 g/kg BW,说明蜂胶薄荷糖属于实际无毒级。遗传毒性试验的试验结果均未显示阳性,表明蜂胶薄荷糖不具有遗传毒性。28 d经口毒性试验表明,试验过程中各组大鼠的生长状况良好,未出现明显中毒体征;大鼠体重、摄食以及血液学和血生化等各项指标均正常;大体解剖检查未观察到被检脏器与蜂胶薄荷糖相关的组织病理学变化,未见有害作用剂量为10 g/kg BW。结论:在该试验研究剂量和条件下,未见蜂胶薄荷糖有毒性作用。Objective:To evaluate the safety and health risk of propolis mints.Methods:Acute oral toxicity test,genotoxicity test(including bacterial reverse mutation test,mammalian erythrocyte micronucleus test and mouse spermatogonia chromosome aberration test)and 28-day oral toxicity test were performed on propolis mints.Results:The acute oral toxicity test showed that no symptoms of poisoning or death were observed in the mice after administration.The median lethal dose(LD50)of propolis mints was>10 g/kg BW,indicating that propolis mints were non-toxic.The results of genotoxicity tests were not positive,indicating that propolis mints are not genotoxic.The 28-day oral toxicity test showed that the rats in each group grew well and had no signs of poisoning during the test.There were no adverse effects on body weight,weight gain,food intake and food utilization rate of rats.Histopathological examination was performed in the main organs of the rats in the high-dose group and the control group,and no histopathological changes were observed in the examined organs related to the test substance,and no harmful effects were observed at the dose of 10 g/kg BW.Conclusion:No toxic effect of propolis mints was observed at the doses and conditions studied in this study.

关 键 词：蜂胶薄荷糖 蜂胶 薄荷脑 安全性试验 毒理学评价 

分 类 号：R285[医药卫生—中药学]