成骨生长肽改性聚醚醚酮表面及其体外成骨活性  

Surface and in vitro osteogenic activity of osteogenic growth peptide-decorated polyetheretherketone

作　　者：杨茗园 余萍萍 李梦琦 张永恒 郑延延 刘吕花 YANG Mingyuan;YU Pingping;LI Mengqi;ZHANG Yongheng;ZHENG Yanyan;LIU Lyuhua(School of Pharmacy,North Sichuan Medical College,Nanchong 637000,China;Department of Clinical Medicine,North Sichuan Medical College,Nanchong 637000,China;School of Stomatology,North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院药学院,四川南充637000 [2]川北医学院临床医学系,四川南充637000 [3]川北医学院口腔医学院,四川南充637000

出　　处：《工程塑料应用》2024年第7期14-21,共8页Engineering Plastics Application

基　　金：四川省科技厅项目(2022YFSY0043,2022NSFSC1537);南充市科技局项目(22SXCXTD0001);四川省大学生创新创业项目(S202310634118)。

摘　　要：为提高聚醚醚酮(PEEK)的成骨活性,首先利用碱性条件下多巴胺(DA)发生氧化自聚合在其表面形成聚多巴胺(PDA)涂层,其次通过PDA将具有成骨活性的成骨生长肽(OGP)化学键合在其表面。通过X射线光电子能谱(XPS)、扫描电子显微镜(SEM)和水接触角测试对改性前后的PEEK表面进行表征。同时,使用成骨细胞MC3T3-E1评价表面未改性和改性PEEK的体外成骨细胞的相容性和成骨活性。XPS结果表明,PEEK表面成功形成PDA涂层,并且通过PDA成功将OGP化学键合在PEEK表面。SEM和水接触角测试结果表明,PDA及OGP改性后PEEK表面形貌均未发生明显变化,但表面亲水性逐渐增强。体外使用成骨细胞MC3T3-E1评价结果显示,与未改性的PEEK相比,PDA及OGP修饰的PEEK表面均改善了成骨细胞MC3T3-E1的黏附、铺展、增殖和成骨分化活性,其中OGP改性的PEEK表面呈现出最佳的成骨细胞黏附、铺展、增殖和成骨分化活性。以上结果表明PEEK表面修饰OGP,可有效提高其成骨细胞相容性和成骨活性,增强了其在骨科等临床领域应用的潜力。In order to improve the osteogenic activity of polyetheretherketone(PEEK),polydopamine(PDA)coating was first formed on its surface by oxidative self-polymerization of dopamine(DA)under alkaline conditions,and then osteogenic growth peptide(OGP)with osteogenic activity was chemically bonded to its surface by PDA.The surfaces of PEEK before and after modification were characterized by X-ray photoelectron spectroscopy(XPS),scanning electron microscopy(SEM)and water contact angle test.Meanwhile,MC3T3-E1 osteoblasts were used to evaluate the in vitro osteoblast compatibility and osteogenic activity of surface unmodified and modified PEEK.The XPS results show that PDA coating is successfully formed on PEEK surface,and OGP is chemically bonded to PEEK surface by PDA.The results of SEM and water contact angle test show that the surface morphology of PEEK after PDA and OGP modification do not change significantly,but the hydrophilicity of PEEK surface after PDA and OGP modification gradually increases.The evaluation results of osteoblasts MC3T3-E1 in vitro show that both PDA and OGP modified PEEK surfaces improve the adhesion,spreading,proliferation and osteogenic differentiation activity of osteoblasts MC3T3-E1 compared with unmodified PEEK.Among them,OGP modified PEEK surface shows the best of osteoblast adhesion,spreading,proliferation and osteogenic differentiation activity.The above results indicate that PEEK surface modified with OGP can effectively improve its osteoblast compatibility and osteogenic activity,and enhancing its potential in clinical fields such as orthopedics.

关 键 词：聚醚醚酮 表面改性 聚多巴胺 成骨生长肽 成骨活性 

分 类 号：R318.08[医药卫生—生物医学工程]