标准全消化道可消化钙、磷含量对断奶仔猪肠-肝-肾轴钙、磷吸收的影响及机制  

Effects and Mechanism of Standard Total Tract Digestibility Calcium andPhosphorus Contents on Calcium and PhosphorusAbsorption in Gut-Liver-Kidney Axis of Weaned Piglets

作　　者：孙焱杰 韦洋洋 苗振彦 熊云霞 吴绮雯 杨雪芬 王丽 蒋宗勇 易宏波 黄艳娜[1] SUN Yanjie;WEI Yangyang;MIAO Zhenyan;XIONG Yunxia;WU QiwenYANG Xuefen;WANG Li;JIANG Zongyong;YI Hongbo;HUANG Yanna(School of Animal Science and Technology,Guangxi University,Nanning 530004,China;Guangdong Key Laboratory of Animal Breeding and Nutrition,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,State Key Laboratory of Swine and Poultry Breeding Industry,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)

机构地区：[1]广西大学动物科学技术学院,南宁530004 [2]广东省农业科学院动物科学研究所,猪禽种业全国重点实验室,农业农村部华南动物营养与饲料重点实验室,广东省畜禽育种与营养研究重点实验室,广州510640

出　　处：《动物营养学报》2024年第7期4243-4257,共15页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家“十四五”重点研发专项(2021YFD1300402);国家现代农业产业技术体系(CAR-35);广东省乡村振兴战略专项资金项目(2023TS-3);科技创新战略专项资金-高水平农科院建设(202106TD,R2023PY-JX014)。

摘　　要：本试验旨在研究标准全消化道可消化钙(STTD Ca)和标准全消化道可消化磷(STTD P)含量对7~25 kg断奶仔猪钙、磷吸收的影响。选取21日龄、体重接近的杜长大杂交断奶仔猪144头,随机分为6组,每组6个重复,每个重复4头猪(阉公猪和母猪各2头)。第1~6组饲粮中STTD Ca和STTD P的比例均为1.2,STTD P含量分别为0.18%、0.26%、0.34%、0.42%、0.50%、0.58%,STTD Ca含量分别为0.216%、0.312%、0.408%、0.504%、0.600%、0.696%。试验期42 d。结果表明:1)随着饲粮中STTD Ca和STTD P的含量逐渐升高,血清碱性磷酸酶活性逐渐降低,但组间没有显著差异(P>0.05),血清降钙素(CT)、甲状腺素(T 4)、1,25二羟维生素D 3[1,25(OH)D 3]、甲状旁腺素(PTH)含量逐渐升高,且6组显著高于1组(P<0.05)。1组血清中钙含量显著低于其他组(P<0.05),其中2、4和6组的血清钙含量较于其他组更高;1组血清磷含量显著高于5组(P<0.05)。2)饲粮中STTD Ca和STTD P的含量逐渐升高对胃内容物及盲肠内容物pH无显著影响(P>0.05)。3)所有组肠道结构完整,1和2组十二指肠绒毛高度/隐窝深度显著高于5和6组(P<0.05),2组空肠绒毛高度/隐窝深度显著低于5组(P<0.05)。4)在十二指肠中,2和3组维生素D受体(VDR)的基因相对表达量显著高于1和5组(P<0.05),1和2组钙磷结合蛋白1(CALB1)的基因相对表达量显著高于4、5和6组(P<0.05),1组细胞色素P450家族成员24A1(CYP24A1)的基因相对表达量显著高于其他组(P<0.05);在空肠中,4和6组的钙离子通道6(TRPV6)的基因相对表达量显著高于2和5组(P<0.05),6组S100钙结合蛋白G(S100G)的基因相对表达量显著高于2组(P<0.05),4组ATP酶质膜钙离子转运蛋白1(ATP2B1)的基因相对表达量显著高于2组(P<0.05),5和6组溶质载体家族蛋白34成员A3(SLC34A3)的基因相对表达量显著高于1和2组(P<0.05),1组CYP24A1的基因相对表达量显著高于其他组(P<0.05);在回肠中,CYP24A1和SLC34A3的基因相对表达量逐渐降低The objective of this study was to investigate the effects of standardized total tract digestibility Ca(STTD Ca)and standardized total tract digestibility P(STTD P)contents on calcium and phosphorus absorption in 7 to 25 kg weaned piglets.A total of 144 weaned piglets with a similar body weight at 21 days of age were randomly divided into 6 groups,with 6 replicates in each group and 4 pigs(two castrated boars and two sows)in each replicate.The proportions of STTD Ca and STTD P in the diet were 1.2,and the contents of STTD P were 0.18%,0.26%,0.34%,0.42%,0.50% and 0.58%,respectively,and the contents of STTD Ca were 0.216%,0.312%,0.408%,0.504%,0.600% and 0.696%,respectively.The trial period was 42 days.The results showed as follows:1)with the gradual increase of the contents of STTD Ca and STTD P in the diet,the serum alkaline phosphatase activity gradually decreased,but there was no significant difference between the groups(P>0.05),and the serum contents of calcitonin(CT),thyroxine(T 4),1,25 dihydroxyvitamin D 3[1,25(OH)D 3]and parathyroid hormone(PTH)increased gradually,and group 6 were significantly higher than group 1(P<0.05).The serum calcium content in group 1 was significantly lower than that in other groups(P<0.05),and the serum calcium content in groups 2,4 and 6 was higher than that in other groups.The serum phosphorus content in group 1 was significantly higher than that in group 5(P<0.05).2)The contents of STTD Ca and STTD P in the diet increased gradually,and there was no significant effect on the pH of gastric contents and cecal contents(P>0.05).3)The intestinal structure of all groups was intact,the duodenal villus height/crypt depth in groups 1 and 2 was significantly higher than that in groups 5 and 6(P<0.05),and the jejunal villus height/crypt depth in group 2 was significantly lower than that in group 5(P<0.05).4)In the duodenum,the relative expression levels of vitamin D receptor(VDR)gene in groups 2 and 3 were significantly higher than those in groups 1 and 5(P<0.05),the relative expression leve

关 键 词：断奶仔猪 STTD Ca STTD P 肠道 肾脏 肝脏 钙、磷吸收 

分 类 号：S828[农业科学—畜牧学]