N-氨甲酰谷氨酸对不同生理阶段湖羊母羊生长性能、血常规指标及血浆抗氧化、免疫指标的影响  

Effects of N-Carbamylglutamic on Growth Performance,Blood Routine Indexes and Plasma Antioxidant and Immune Indexes of Female Hu Sheep at Different Physiological Stages

作　　者：杨兴林 丁常根 李金昕 郭志伟 陈佳佳 李陇平[1] 屈雷[1] YANG Xinglin;DING Changgen;LI Jinxin;GUO Zhiwei;CHEN Jiajia;LI Longping;QU Lei(Shaanxi Cashmere Goat Engineering Technology Research Center,Yulin University,Yulin 719000,China;College of Life Sciences,Yulin University,Yulin 719000,China)

机构地区：[1]榆林学院,陕西省绒山羊工程技术研究中心,榆林719000 [2]榆林学院生命科学学院,榆林719000

出　　处：《动物营养学报》2024年第7期4473-4485,共13页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：榆林市科技局产学研项目(2023-CXY-174);榆林市科技局产学研项目(CXY-2022-54)。

摘　　要：本试验旨在研究妊娠期、哺乳期和空怀期饲粮中添加不同水平N-氨甲酰谷氨酸(NCG)对湖羊母羊生长性能、血常规指标及血浆抗氧化、免疫指标的影响,为NCG在湖羊母羊整个生产周期不同生理阶段中的应用提供依据。选择300只9月龄、体重[(33.6±1.2)kg]相近、健康的湖羊初产母羊,随机分为4组,每组5个重复,每个重复15只。采用本交方式配种1个月后,通过B超检测每组选择40只妊娠母羊,每组4个重复,每个重复10只。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加1.0(Ⅰ组)、1.5(Ⅱ组)和2.0 g/d(Ⅲ组)的NCG。试验从母羊配种前开始至羔羊断奶7 d后结束。分别在妊娠前期(公羊配种结束后28 d)、妊娠后期(预产期前15 d)、哺乳期(产后7 d)、空怀期(羔羊断奶后7 d)采集不同生理阶段的母羊血液,测定血常规指标及血浆抗氧化、免疫指标。结果表明:1)在妊娠前期,试验Ⅱ组血液单核细胞数量、平均血红蛋白含量显著高于对照组(P<0.05),试验Ⅰ组血液平均血红蛋白浓度显著高于对照组(P<0.05),试验Ⅰ组血浆总抗氧化能力显著高于对照组(P<0.05)。2)在妊娠后期,试验Ⅲ组血液血小板平均体积显著高于对照组(P<0.05),试验Ⅲ组血浆丙二醛含量极显著低于对照组(P<0.01),试验Ⅲ组血浆白细胞介素-10含量显著高于对照组(P<0.05),试验Ⅰ组血浆肿瘤坏死因子-α含量显著低于对照组(P<0.05)。3)在哺乳期,试验Ⅱ组血浆超氧化物歧化酶活性显著高于对照组(P<0.05),试验Ⅲ组血浆丙二醛含量显著低于对照组(P<0.05),试验Ⅲ组血浆总抗氧化能力显著高于对照组(P<0.05),试验Ⅰ组血浆肿瘤坏死因子-α含量显著低于对照组(P<0.05)。4)在空怀期,试验Ⅰ组血液红细胞体积显著高于对照组(P<0.05),试验Ⅰ组和试验Ⅱ组血液平均血红蛋白含量显著高于对照组(P<0.05),试验Ⅰ组血浆肿瘤坏死因子-α含量极显著低于对照组(P<0.01)�This experiment was conducted to investigate the effects of dietary different supplemental levels of N-carbamylglutamic(NCG)at pregnancy period,lactation period and empty period on growth performance,blood routine indexes and plasma antioxidant and immune indexes of female Hu sheep at different physiological stages,and to provide basis for the application of NCG in different physiological stages of the whole production cycle of ewe.A total of 300 healthy first bearing female Hu sheep with similar body weight[(33.6±1.2)kg]at 9 months of age were randomly divided into 4 groups with 5 replicates in each group and 15 sheep in each group.After one month of breeding with natural service method,40 pregnant ewes in each group were selected by B-ultrasound detection,with 4 replicates in each group and 10 sheep in each group.The control group was fed a basal diet,and the experimental groups were fed the basal diet supplemented with 1.0(groupⅠ),1.5(groupⅡ)and 2 g/d(groupⅢ)NCG,respectively.The experiment began before the ewes were mated and ended 7 days after the lambs were weaned.The blood of ewes at different physiological stages were collected at early pregnancy period(28 days after the end of male breeding),late pregnancy period(15 days before the expected date of delivery),lactation period(7 days postpartum)and empty period(7 days after weaning of lambs),and determined the blood routine indexes and plasma antioxidant and immune indexes.The results showed as follows:1)at early pregnancy period,the blood monocyte number and mean corpuscular hemoglobin content of trial groupⅡwere significantly higher than those of the control group(P<0.05),the blood mean corpuscular hemoglobin concentration of trial groupⅠwas significantly higher than that of the control group(P<0.05),and the plasma total antioxidant capacity of trial groupⅠwas significantly higher than that of the control group(P<0.05).2)At late pregnancy period,the blood mean platelet volume of trial groupⅢwas significantly higher than that of the control

关 键 词：N-氨甲酰谷氨酸 湖羊 血常规 抗氧化 免疫 

分 类 号：S826[农业科学—畜牧学]