针刺大鼠肺俞三穴对毒蕈碱性胆碱受体M2在哮喘中的作用  

Effects of Needling BL13,EX-B1 and RN17 on CHRM2 Receptor in Rats with Asthma

作　　者：樊雅哲 刘玉丽[1] FAN Yazhe;LIU Yuli(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《针灸临床杂志》2024年第8期67-71,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金,编号:81070011;辽宁省自然科学基金,编号:2021-MS-247。

摘　　要：目的:探讨针刺肺俞、定喘和膻中三穴对支气管哮喘大鼠肺组织中毒蕈碱型胆碱受体M2(CHRM2)的影响。方法:6~8周龄Wistar大鼠被分为正常对照组、模型组、针刺组、肺俞穴组和非经非穴组,每组10只。应用卵蛋白(OVA)致敏、诱导建立支气管哮喘大鼠模型,肺功能仪检测肺功能,苏木精-伊红(HE)染色法观察大鼠肺组织病理改变,免疫荧光检测CHRM2蛋白表达变化,Western blot蛋白质印迹法测定肺组织中毒蕈碱型胆碱受体M2蛋白含量。结果:大鼠肺功能检测显示针刺肺俞、定喘与膻中穴能明显降低哮喘组升高的气道阻力、降低气道炎性反应;病理显示模型组支气管炎性细胞浸润增加。免疫荧光显示针刺组CHRM2蛋白表达水平与模型组比较明显降低,差异具有统计学意义(P<0.01);Western blot结果显示针刺组大鼠CHRM2蛋白表达水平与模型组比较明显降低,差异具有统计学意义(P<0.01)。结论:针刺肺俞、定喘与膻中穴降低CHRM2水平可降低哮喘升高的气道高反应。Objective:To investigate the effects of needling Feishu(BL13),Dingchuan(EX-B1)and Danzhong(RN17)on cholinergic receptor muscarinic 2(CHRM2)receptor in lung tissues of the rats with bronchial asthma.Methods:50 Wistar rats(6-8 weeks)were randomly divided into the normal control group,the model group,the acupuncture group,the BL13 group and the non-meridian and non-acupuncture group,with 10 rats in each group.Ovalbumin(OVA)was applied to sensitize and induce the model establishment of bronchial asthma.Lung function was detected by pulmonary function meter,pathological changes in lung tissues were observed by HE staining,changes in CHRM2 protein were detected by immunofluorescence,and CHRM2 protein content in lung tissues was determined by Western Blot.Results:Lung function test in rats showed that needling BL13,EX-B1 and RN17 could significantly reduce the increased airway resistance to reduce the airway inflammatory reaction in the rats with bronchial asthma.Pathology showed that bronchial inflammatory cell infiltration was increased in the model group.Immunofluorescence showed that the protein expressions of CHRM2 were significantly lower in the acupuncture group than those in the model group(P<0.01).Western Blot showed that the protein expressions of CHRM2 were significantly lower in the acupuncture group than those in the model group(P<0.01).Conclusion:Needling BL13,EX-B1 and RN17 can reduce the increased airway hyperresponsiveness in asthma rats by lowering CHRM2 levels.

关 键 词：哮喘 针刺 定喘 CHRM2受体 

分 类 号：R246.1[医药卫生—针灸推拿学]