天祝白牦牛ADIPOQ基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of the ADIPOQ gene in Tianzhu White yak

作　　者：若周么 石斌刚 王向彦 祁有鹏 胡江 RUO Zhoume;SHI Bin’gang;WANG Xiangyan;QI Youpeng;HU Jiang(Agricultural and Animal Husbandry Technology Service Station in Dawu Town of Maqin County,MaQin 814000,China;Gansu Key Laboratory of Herbivorous Animal Biotechnology,College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]玛沁县大武镇农牧业技术服务站,青海玛沁814000 [2]甘肃农业大学动物科学技术学院甘肃省草食动物生物技术重点实验室,兰州730070

出　　处：《黑龙江畜牧兽医》2024年第13期105-110,115,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：少数民族专业技术人才特殊培养计划项目(033-02000102);祁连山牧区牦牛肉牛农牧循环高效生产技术研发与示范项目(2022CYZC43)。

摘　　要：脂联素(ADIPOQ)是一种主要由脂肪组织分泌的内源性细胞因子,在调节脂类代谢中发挥重要作用,该基因通过调控脂质代谢影响动物机体脂肪沉积。为探讨ADIPOQ基因编码蛋白序列特征及组织表达模式,试验对天祝白牦牛ADIPOQ基因编码序列(CDS)进行克隆测序,获得天祝白牦牛ADIPOQ基因CDS区,利用生物信息学软件预测天祝白牦牛ADIPOQ基因及其编码氨基酸序列特征。结果表明:天祝白牦牛ADIPOQ基因CDS区全长723 bp,编码240个氨基酸,其中甘氨酸含量(14.17%)最高,具有亲水性,蛋白质二级结构和三级结构以无规则卷曲和延伸链为主。ADIPOQ基因编码蛋白具有一个信号肽,无跨膜结构,属于分泌蛋白,其可能与ADIPOR1、ADIPORZ、RETN、PLIN1和LEP等10个蛋白质存在相互作用,与山羊的氨基酸序列相似性为91.6%,与绵羊、山羊亲缘关系最近,具有一定的保守性。说明试验成功克隆了天祝白牦牛ADIPOQ基因的CDS区,ADIPOQ基因可能在脂肪沉积过程中发挥重要作用。Lipocalin(ADIPOQ)is an endogenous cytokine secreted mainly by adipose tissue,which plays an important role in the regulation of lipid metabolism,and it affects fat deposition in animal organisms by regulating lipid metabolism.In order to explore the protein sequence characteristics and tissue expression pattern of ADIPOQ gene,the CDS region of ADIPOQ gene of Tianzhu White yak was cloned by Sanger sequencing,and the CDS sequence of ADIPOQ gene of Tianzhu White yak was obtained;and bioinformatics softwares were used to predict the characteristics of ADIPOQ gene and its amino acid sequence of Tianzhu White yak.The results showed that the CDS region of the ADIPOQ gene of Tianzhu White yak was 723 bp in length;it encoded 240 amino acids,of which glycine was the most abundant;and the secondary and tertiary structure of the protein was dominated by irregularly coiled and extended chains.The protein encoded by ADIPOQ gene had a signal peptide without transmembrane structure,and belonged to hydrophilic stable protein.In this experiment,the sequence of the CDS region of the ADIPOQ gene of the Tianzhu White yak was successfully cloned;ADIPOQ gene might play an important role in fat deposition.

关 键 词：天祝白牦牛 ADIPOQ基因 克隆 CDS区 生物信息学分析 

分 类 号：S813.3[农业科学—畜牧学] S823.8*5[农业科学—畜牧兽医]