大豆遗传图谱构建及粗脂肪含量QTL定位  

Construction of Genetic Map and QTL Mapping of Crude Fat Content in Soybean

作　　者：李熙 易靖 熊郭烯 马蓉寒 高伟然 孙浩 刘佳琦 姜澳华 方小梅 易泽林[1] 张建[1] LI Xi;YI Jing;XIONG Guoxi;MA Ronghan;GAO Weiran;SUN Hao;LIU Jiaqi;JIANG Aohua;FANG Xiaomei;YI Zelin;ZHANG Jian(School of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China;Chongqing Agro-Tech Extension Station,Chongqing 401121,China)

机构地区：[1]西南大学农学与生物科技学院,重庆400715 [2]重庆市农业技术推广总站,重庆401121

出　　处：《西南大学学报（自然科学版）》2024年第8期12-21,共10页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金面上项目(31971984);重庆市大学生创新创业训练计划资助项目(202310635404);重庆市技术创新与应用发展专项重点项目(cstc2021jscx-gksbX0011);重庆市科企联合体种质资源收集利用与品种试验项目(cqnyncw-kqlhtxm).

摘　　要：大豆是我国重要的粮油作物,提高其粗脂肪含量是我国大豆育种的重要目标.以长江春2号与渝蜀鲜2号杂交的F 2代186株单株为定位群体,在实验室前期构建的遗传图谱基础上进行标记加密,利用Joinmap 4.0软件进行遗传连锁分析,构建了包含480个标记的大豆遗传图谱,总长度为2469.3 cM,标记间平均距离为5.14 cM.结合大豆在2021年重庆、2022年重庆、2022年云南和2023年重庆4个环境下的粗脂肪含量表型数据,通过区间作图(Interval Mapping,IM)检测相应性状的数量性状位点(Quantitative Trait Locus,QTL).在4个环境中共检测到21个与粗脂肪含量相关的QTLs,分布在16个连锁群上,其中,有3个QTLs(qOIL01.1,qOIL04.1和qOIL14.2)在两个环境中均能检测到.Soybean is an important grain and oil crop,so increasing crude fat content of soybean seed is an important goal of soybean breeding in China.Based on the genetic map constructed in the laboratory,the density of the marker was increased.By using Joinmap 4.0 software,a soybean genetic map containing 480 markers with an average distance of 5.14 cM and a total length of 2469.3 cM was obtained.Combined with the phenotypic data of crude fat content of soybean in Chongqing in 2021,2022 and 2023,Yunnan in 2022,QTLs(quantitative trait locus)were detected by IM mapping(interval mapping).Twenty-one QTLs related to the crude fat content of soybean were obtained under four environmental conditions,distributed on 16 linkage groups,among which,three QTLs(qOIL01.1,qOIL04.1 and qOIL14.2)were detected in two environments.The research results will lay the foundation of map-based cloning and gene functional analysis for QTL affecting crude oil content in soybean.

关 键 词：大豆 粗脂肪含量 遗传图谱 数量性状位点 

分 类 号：Q943.2[生物学—植物学] S565.1[农业科学—作物学]