鸭瘟病毒载体疫苗研究进展  

Research Progress of Duck Plague Virus Vector Vaccines

作　　者：封莹洁 孟鸽 房立春 罗娟 尚佳静 于晓慧 蒋文明[2] 刘华雷[2] 范春艳 李阳 Feng Yingjie;Meng Ge;Fang Lichun;Luo Juan;Shang Jiajing;Yu Xiaohui;Jiang Wenming;Liu Hualei;Fan Chunyan;Li Yang(College of Life Sciences and Food Engineering,Hebei University of Engineering,Handan 056038,Hebei,China;China Animal Health and Epidemiology Center,Qingdao 266032,Shandong,China;Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250000,Shandong,China;Qingdao Key Laboratory of Modern Bioengineering and Animal Disease Research,Qingdao 266032,Shandong,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,河北邯郸056038 [2]中国动物卫生与流行病学中心,山东青岛266032 [3]山东省农业科学院畜牧兽医研究所,山东济南250000 [4]青岛市现代生物工程及动物疫病研究重点实验室,山东青岛266032

出　　处：《中国动物检疫》2024年第7期61-68,共8页China Animal Health Inspection

基　　金：泰山学者工程资助项目(tsqnz20221159)。

摘　　要：疫苗接种是控制疫病的有效方法一,其中病毒载体疫苗以其转染效率高、外源基因表达水平高等优点成为当前疫苗的重要研究方向。鸭瘟病毒基因非常适合作为插入和表达其他病原体的外源抗原基因,并且随着CRISPR/Cas9技术、细菌人工染色体技术、同源重组技术等基因编辑技术的发展,鸭瘟病毒作为最有前途的载体被广泛应用于传染病疫苗研发。经试验验证,基于重组鸭瘟病毒载体的禽细菌病疫苗以及禽流感、鸭病毒性肝炎等多种病毒病疫苗均具有较好的免疫效果和安全性。本文综述了鸭瘟病毒作为疫苗载体的特点、鸭瘟病毒的基因编辑技术和鸭瘟病毒载体疫苗应用研究进展情况,以期为进一步研发鸭瘟病毒载体疫苗提供理论基础,同时也为新发传染病预防提供新策略。Vaccination is one of effective methods to control animal diseases,especially viral vector vaccines with advantages of high transfection efficiency and high expression level of exogenous genes,which is becoming an important research direction at present.Duck plague virus genome is very suitable to insert and express exogenous genes of other pathogens,and has been widely used in the development of infectious disease vaccines as the most promising virus vector with the development of gene editing technologies such as CRISPR/Cas9,bacterial artificial chromosome technology,homologous recombination technology,etc.As verified by experiments,good immunization effect and safety have been detected in avian bacterial disease vaccines based on recombinant duck plague virus vectors as well as viral disease vaccines such as avian infuenza vaccine and duck viral hepatitis vaccine.In the paper,the characteristics of duck plague virus as a vaccine vector as well as research progress on its gene editing technologies and application of the vector vaccines were reviewed,aiming to provide a theoretical basis for further research and development of such vaccines,and a new strategy for future prevention of any emerging infectious diseases.

关 键 词：鸭瘟病毒 病毒载体疫苗 基因编辑技术 疫苗研发 

分 类 号：S852.65[农业科学—基础兽医学]